Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhizomucor miehei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.17 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 50°C | Rhizomucor miehei | |
0.92 | - |
cellotetraose | pH 5.0, 50°C | Rhizomucor miehei | |
1.26 | - |
laminaribiose | pH 5.0, 50°C | Rhizomucor miehei | |
1.5 | 2 | cellotriose | pH 5.0, 50°C | Rhizomucor miehei | |
2.29 | - |
laminaritriose | pH 5.0, 50°C | Rhizomucor miehei | |
2.32 | - |
Laminaran | pH 5.0, 50°C | Rhizomucor miehei | |
3.7 | - |
cellobiose | pH 5.0, 50°C | Rhizomucor miehei | |
4.15 | - |
gentobiose | pH 5.0, 50°C | Rhizomucor miehei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66400 | - |
gel filtration | Rhizomucor miehei |
76500 | - |
SDS-PAGE | Rhizomucor miehei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizomucor miehei | A0A0B4RUW2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.1 | - |
substrate laminaran, pH 5.0, 50°C | Rhizomucor miehei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + D-glucose | 2.7% of the activity with 4-nitrophenyl alpha-D-glucopyranoside | ? | |
4-nitrophenyl alpha-D-xylopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + D-xylose | 3.9% of the activity with 4-nitrophenyl alpha-D-glucopyranoside | ? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Rhizomucor miehei | 4-nitrophenol + D-glucose | - |
? | |
cellobiose + H2O | - |
Rhizomucor miehei | 2 D-glucose | 62% of the activity with laminaribiose | ? | |
cellotetraose + H2O | - |
Rhizomucor miehei | D-glucose + ? | 64% of the activity with laminaribiose | ? | |
cellotriose + H2O | - |
Rhizomucor miehei | D-glucose + ? | 67% of the activity with laminaribiose | ? | |
gentobiose + H2O | - |
Rhizomucor miehei | 2 D-glucose | 52% of the activity with laminaribiose | ? | |
laminaran + H2O | 67% of the activity with laminaribiose | Rhizomucor miehei | D-glucose + gentiobiose + gentiotriose | - |
? | |
laminaribiose + H2O | - |
Rhizomucor miehei | 2 D-glucose | - |
? | |
laminaritriose + H2O | - |
Rhizomucor miehei | D-glucose + ? | 97% of the activity with laminaribiose | ? | |
additional information | isoform Bglu3B exhibits a broad range of substrate specificity, catalyzing the cleavage of beta-1,2, beta-1,3, beta-1,4 and beta-1,6 linkages, in various oligosaccharides, to liberate glucose, e.g. reaction of EC 3.2.1.58. Bglu3B shows relatively high activity toward laminaran and transglycosylation activity, enabling gentiobiose production. No substrates: 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, lactose, barley beta-glucan, Avicel, carboxymethyl cellulose, lichenan, birchwood xylan and chitin | Rhizomucor miehei | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Bglu3B | - |
Rhizomucor miehei |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhizomucor miehei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
Laminaran | pH 5.0, 50°C | Rhizomucor miehei | |
0.043 | - |
cellobiose | pH 5.0, 50°C | Rhizomucor miehei | |
0.056 | - |
gentobiose | pH 5.0, 50°C | Rhizomucor miehei | |
0.059 | - |
cellotetraose | pH 5.0, 50°C | Rhizomucor miehei | |
0.067 | - |
cellotriose | pH 5.0, 50°C | Rhizomucor miehei | |
0.071 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 50°C | Rhizomucor miehei | |
0.077 | - |
laminaritriose | pH 5.0, 50°C | Rhizomucor miehei | |
0.078 | - |
laminaribiose | pH 5.0, 50°C | Rhizomucor miehei |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rhizomucor miehei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
cellobiose | pH 5.0, 50°C | Rhizomucor miehei | |
0.013 | - |
gentobiose | pH 5.0, 50°C | Rhizomucor miehei | |
0.014 | - |
Laminaran | pH 5.0, 50°C | Rhizomucor miehei | |
0.033 | - |
laminaritriose | pH 5.0, 50°C | Rhizomucor miehei | |
0.044 | - |
cellotriose | pH 5.0, 50°C | Rhizomucor miehei | |
0.062 | - |
laminaribiose | pH 5.0, 50°C | Rhizomucor miehei | |
0.063 | - |
cellotetraose | pH 5.0, 50°C | Rhizomucor miehei | |
0.42 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 50°C | Rhizomucor miehei |