Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-fucose | 10 mM, 68% inhibition, competitive | Thermothelomyces thermophilus | |
D-gluconolactone | 10 mM, 80% inhibition, competitive | Thermothelomyces thermophilus | |
D-glucose | 10 mM, 80% inhibition, competitive | Thermothelomyces thermophilus | |
maltose | 10 mM, 65% inhibition, competitive | Thermothelomyces thermophilus | |
Nojirimycin | 10 mM, 80% inhibition, competitive | Thermothelomyces thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
4-nitrophenyl beta-D-glucose | pH 5.4, 50°C | Thermothelomyces thermophilus | |
0.35 | - |
gentiobiose | pH 5.4, 50°C | Thermothelomyces thermophilus | |
0.83 | - |
cellobiose | pH 5.4, 50°C | Thermothelomyces thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermothelomyces thermophilus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermothelomyces thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
after growth on cellulose | Thermothelomyces thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
89 | - |
substrate 4-nitrophenyl beta-D-glucose, pH 5.4, 50°C | Thermothelomyces thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucose + H2O | - |
Thermothelomyces thermophilus | 4-nitrophenyl + D-glucose | - |
? | |
cellobiose + H2O | - |
Thermothelomyces thermophilus | 2 D-glucose | - |
? | |
cellohexaose + 5 H2O | - |
Thermothelomyces thermophilus | 6 D-glucose | - |
? | |
cellopentaose + 4 H2O | - |
Thermothelomyces thermophilus | 5 D-glucose | - |
? | |
cellotetraose + 3 H2O | - |
Thermothelomyces thermophilus | 4 D-glucose | - |
? | |
cellotriose + 2 H2O | - |
Thermothelomyces thermophilus | 3 D-glucose | - |
? | |
esculin + H2O | - |
Thermothelomyces thermophilus | esculetin + beta-D-glucose | - |
? | |
gentiobiose + H2O | - |
Thermothelomyces thermophilus | 2 D-glucose | - |
? | |
laminaribiose + H2O | - |
Thermothelomyces thermophilus | 2 D-glucose | - |
? | |
additional information | above 1 mM substrate concentration, enzyme catalyses a parallel transglycosylation reaction. No substrate: beta-1,4-glucan | Thermothelomyces thermophilus | ? | - |
? | |
salicin + H2O | - |
Thermothelomyces thermophilus | D-glucose + salicyl alcohol | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 5.4, stable for 6 h | Thermothelomyces thermophilus |
60 | - |
2 h, 25% loss of activity | Thermothelomyces thermophilus |
70 | - |
complete loss of activity within 1 h | Thermothelomyces thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
- |
Thermothelomyces thermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | 50°C, stable for at least 6 h | Thermothelomyces thermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00026 | - |
Nojirimycin | pH 5.4, 50°C | Thermothelomyces thermophilus | |
0.008 | - |
D-gluconolactone | pH 5.4, 50°C | Thermothelomyces thermophilus | |
0.5 | - |
D-glucose | pH 5.4, 50°C | Thermothelomyces thermophilus |