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Literature summary for 3.2.1.21 extracted from

  • Bhat, K.; Gaikwad, J.; Maheshwari, R.
    Purification and characterization of an extracellular beta-glucosidase from the thermophilic fungus Sporotrichum thermophile and its influence on cellulase activity (1993), J. Gen. Microbiol., 139, 2825-2832 .
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
D-fucose 10 mM, 68% inhibition, competitive Thermothelomyces thermophilus
D-gluconolactone 10 mM, 80% inhibition, competitive Thermothelomyces thermophilus
D-glucose 10 mM, 80% inhibition, competitive Thermothelomyces thermophilus
maltose 10 mM, 65% inhibition, competitive Thermothelomyces thermophilus
Nojirimycin 10 mM, 80% inhibition, competitive Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
4-nitrophenyl beta-D-glucose pH 5.4, 50°C Thermothelomyces thermophilus
0.35
-
gentiobiose pH 5.4, 50°C Thermothelomyces thermophilus
0.83
-
cellobiose pH 5.4, 50°C Thermothelomyces thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermothelomyces thermophilus
-
-

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
after growth on cellulose Thermothelomyces thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
89
-
substrate 4-nitrophenyl beta-D-glucose, pH 5.4, 50°C Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucose + H2O
-
Thermothelomyces thermophilus 4-nitrophenyl + D-glucose
-
?
cellobiose + H2O
-
Thermothelomyces thermophilus 2 D-glucose
-
?
cellohexaose + 5 H2O
-
Thermothelomyces thermophilus 6 D-glucose
-
?
cellopentaose + 4 H2O
-
Thermothelomyces thermophilus 5 D-glucose
-
?
cellotetraose + 3 H2O
-
Thermothelomyces thermophilus 4 D-glucose
-
?
cellotriose + 2 H2O
-
Thermothelomyces thermophilus 3 D-glucose
-
?
esculin + H2O
-
Thermothelomyces thermophilus esculetin + beta-D-glucose
-
?
gentiobiose + H2O
-
Thermothelomyces thermophilus 2 D-glucose
-
?
laminaribiose + H2O
-
Thermothelomyces thermophilus 2 D-glucose
-
?
additional information above 1 mM substrate concentration, enzyme catalyses a parallel transglycosylation reaction. No substrate: beta-1,4-glucan Thermothelomyces thermophilus ?
-
?
salicin + H2O
-
Thermothelomyces thermophilus D-glucose + salicyl alcohol
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
pH 5.4, stable for 6 h Thermothelomyces thermophilus
60
-
2 h, 25% loss of activity Thermothelomyces thermophilus
70
-
complete loss of activity within 1 h Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
Thermothelomyces thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6.5 50°C, stable for at least 6 h Thermothelomyces thermophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00026
-
Nojirimycin pH 5.4, 50°C Thermothelomyces thermophilus
0.008
-
D-gluconolactone pH 5.4, 50°C Thermothelomyces thermophilus
0.5
-
D-glucose pH 5.4, 50°C Thermothelomyces thermophilus