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Literature summary for 3.2.1.21 extracted from

  • Matsuzawa, T.; Jo, T.; Uchiyama, T.; Manninen, J.; Arakawa, T.; Miyazaki, K.; Fushinobu, S.; Yaoi, K.
    Crystal structure and identification of a key amino acid for glucose tolerance, substrate specificity, and transglycosylation activity of metagenomic beta-glucosidase Td2F2 (2016), FEBS J., 283, 2340-2353 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
in ligand-free form, and in complex form with D-glucose, with inhibitor isofagomine, and D-fucose, wild-type and mutants in residue N223, to 1.2-1.9 A resolution uncultured microorganism

Protein Variants

Protein Variants Comment Organism
N223Q mutation results in reduced glucose tolerance and transglycosylation activity, and drastically changed substrate specificity uncultured microorganism
N223Y mutation results in reduced glucose tolerance and transglycosylation activity, and drastically changed substrate specificity uncultured microorganism

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.14
-
4-nitrophenyl beta-D-fucopyranoside wild-type, pH 5.5, 75°C uncultured microorganism
0.21
-
4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.5, 75°C uncultured microorganism
0.39
-
4-nitrophenyl beta-D-fucopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
0.71
-
4-nitrophenyl beta-D-galactopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
0.85
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
2.65
-
4-nitrophenyl beta-D-glucopyranoside wild-type, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism
3.61
-
4-nitrophenyl beta-D-galactopyranoside wild-type, pH 5.5, 75°C uncultured microorganism
3.99
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Y, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism
5
-
4-nitrophenyl beta-D-fucopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
7.69
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
17.6
-
4-nitrophenyl beta-D-galactopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
34.3
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Q, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism

Organism

Organism UniProt Comment Textmining
uncultured microorganism A0A1E1FFN6 isolated from a compost metagenome
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-fucopyranoside + H2O
-
uncultured microorganism 4-nitrophenol + D-fucose
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
uncultured microorganism 4-nitrophenol + D-galactose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
uncultured microorganism 4-nitrophenol + D-glucose
-
?

Synonyms

Synonyms Comment Organism
Td2F2
-
uncultured microorganism

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
assay at uncultured microorganism

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.94
-
4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.5, 75°C uncultured microorganism
16
-
4-nitrophenyl beta-D-fucopyranoside wild-type, pH 5.5, 75°C uncultured microorganism
17.6
-
4-nitrophenyl beta-D-galactopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
30.8
-
4-nitrophenyl beta-D-galactopyranoside wild-type, pH 5.5, 75°C uncultured microorganism
32.3
-
4-nitrophenyl beta-D-galactopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
36.7
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
38.7
-
4-nitrophenyl beta-D-fucopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
42.5
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
69
-
4-nitrophenyl beta-D-glucopyranoside wild-type, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism
80.4
-
4-nitrophenyl beta-D-fucopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
82.8
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Q, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism
145
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Y, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at uncultured microorganism

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.83
-
4-nitrophenyl beta-D-galactopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
2.41
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Q, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism
5.53
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
8.52
-
4-nitrophenyl beta-D-galactopyranoside wild-type, pH 5.5, 75°C uncultured microorganism
16.1
-
4-nitrophenyl beta-D-fucopyranoside mutant N223Q, pH 5.5, 75°C uncultured microorganism
24.7
-
4-nitrophenyl beta-D-galactopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
26.1
-
4-nitrophenyl beta-D-glucopyranoside wild-type, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism
32.3
-
4-nitrophenyl beta-D-glucopyranoside wild-type, pH 5.5, 75°C uncultured microorganism
36.4
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Y, presence of 500 mM D-glucose, pH 5.5, 75°C uncultured microorganism
43.2
-
4-nitrophenyl beta-D-glucopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
98.3
-
4-nitrophenyl beta-D-fucopyranoside mutant N223Y, pH 5.5, 75°C uncultured microorganism
115
-
4-nitrophenyl beta-D-fucopyranoside wild-type, pH 5.5, 75°C uncultured microorganism