Any feedback?
Literature summary for 3.2.1.21 extracted from
- Characterization of a recombinant thermostable beta-glucosidase from Putranjiva roxburghii expressed in Saccharomyces cerevisiae and its use for efficient biomass conversion (2017), Process Biochem., 63, 66-75 .
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| degradation | use for bioethanol production from different cellulosic biomass sources. Using simultaneous saccharification and fermentation, 9.47 g/l and 14.32 g/l of bioethanol can be obtained from carboxymethyl cellulose and pretreated rice straw, respectively | Putranjiva roxburghii |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae | Putranjiva roxburghii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
4-nitrophenyl beta-D-fucopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii |  |
| 0.52 | - |
4-nitrophenyl beta-D-glucopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 0.65 | - |
4-nitrophenyl beta-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 0.77 | - |
cellobiose | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 2.01 | - |
4-nitrophenyl beta-D-cellobioside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Putranjiva roxburghii | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Putranjiva roxburghii | A0A023MIF8 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Putranjiva roxburghii |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Putranjiva roxburghii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 119 | - |
substrate beta-D-glucopyranoside, pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-D-galactopyranoside + H2O | - |
Putranjiva roxburghii | 4-methylumbelliferone + beta-D-galactose | - |
? | |
| 4-methylumbelliferyl beta-D-glucopyranoside + H2O | - |
Putranjiva roxburghii | 4-methylumbelliferone + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-cellobioside + H2O | - |
Putranjiva roxburghii | 4-nitrophenol + cellobiose | - |
? | |
| 4-nitrophenyl beta-D-fucopyranoside + H2O | - |
Putranjiva roxburghii | 4-nitrophenol + beta-D-fucose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Putranjiva roxburghii | 4-nitrophenol + beta-D-galactose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Putranjiva roxburghii | 4-nitrophenol + beta-D-glucose | - |
? | |
| amygdalin + 2 H2O | - |
Putranjiva roxburghii | mandelonitrile + 2 D-glucose | - |
? | |
| arbutin + H2O | - |
Putranjiva roxburghii | 4-hydroxyphenol + D-glucose | - |
? | |
| cellobiose + H2O | - |
Putranjiva roxburghii | 2 D-glucose | - |
? | |
| salicin + H2O | - |
Putranjiva roxburghii | saligenin + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68000, SDS-PAGE | Putranjiva roxburghii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Putranjiva roxburghii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
stable for 60 min | Putranjiva roxburghii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.56 | - |
4-nitrophenyl beta-D-cellobioside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 7.88 | - |
cellobiose | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 7.95 | - |
4-nitrophenyl beta-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 11.7 | - |
4-nitrophenyl beta-D-glucopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 14.05 | - |
4-nitrophenyl beta-D-fucopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Putranjiva roxburghii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.76 | - |
4-nitrophenyl beta-D-cellobioside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 10.2 | - |
cellobiose | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 12.2 | - |
4-nitrophenyl beta-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 22.6 | - |
4-nitrophenyl beta-D-glucopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
| 29.9 | - |
4-nitrophenyl beta-D-fucopyranoside | pH not specified in the publication, temperature not specified in the publication | Putranjiva roxburghii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
