Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Agrobacterium tumefaciens |
Crystallization (Comment) | Organism |
---|---|
molecular modeling of structure | Agrobacterium tumefaciens |
Protein Variants | Comment | Organism |
---|---|---|
C174V | contrary to uncompetitive inhibition of wild-type by glucose, mutant is competitively inhibited | Agrobacterium tumefaciens |
H229S | contrary to uncompetitive inhibition of wild-type by glucose, mutant is competitively inhibited | Agrobacterium tumefaciens |
L178E | mutant shows enhanced thermostability | Agrobacterium tumefaciens |
L178E | specific activity is1.8 times increased on substrate cellobiose | Agrobacterium tumefaciens |
V176A | slight increase in specific activity | Agrobacterium tumefaciens |
W127F | mutant shows enhanced thermostability | Agrobacterium tumefaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glucose | - |
Agrobacterium tumefaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.85 | - |
cellobiose | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
2.61 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
2.7 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
2.9 | - |
cellobiose | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
3.1 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
5 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
5.5 | - |
cellobiose | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
6.8 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
7 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.5 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
8 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
8.3 | - |
cellobiose | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
8.5 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
10.1 | - |
cellobiose | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
10.14 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.25 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
12.5 | - |
cellobiose | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
13.3 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
19.6 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
22.4 | - |
cellobiose | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Agrobacterium tumefaciens | H0HC94 | - |
- |
Agrobacterium tumefaciens 5A | H0HC94 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
204 | - |
wild-type, substrate cellobiose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens |
248 | - |
wild-type, substrate 4-nitrophenyl beta-D-glucopyranoside, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens | nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Agrobacterium tumefaciens 5A | nitrophenol + D-glucose | - |
? | |
cellobiose + H2O | - |
Agrobacterium tumefaciens | 2 D-glucose | - |
? | |
cellobiose + H2O | - |
Agrobacterium tumefaciens 5A | 2 D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AT5A_15831 | - |
Agrobacterium tumefaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
46 | - |
mutant C174W | Agrobacterium tumefaciens |
52 | - |
wild-type, mutants V176A, L178A, H229S | Agrobacterium tumefaciens |
53 | - |
mutant L178E | Agrobacterium tumefaciens |
55 | - |
mutant W127F | Agrobacterium tumefaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
91 | - |
cellobiose | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
95 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
107 | - |
cellobiose | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
118 | - |
cellobiose | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
131.5 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
163 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.5 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
193 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
230 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.25 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
233 | - |
cellobiose | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
277 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
282 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
324 | - |
cellobiose | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
328 | - |
cellobiose | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
334.9 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
354 | - |
cellobiose | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
363 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
390 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
559 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
mutant W127F | Agrobacterium tumefaciens |
7 | - |
mutants C174V, V176A, L178A, L178E, H229S | Agrobacterium tumefaciens |
7.2 | - |
wild-type | Agrobacterium tumefaciens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
518 | - |
D-glucose | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
558 | - |
D-glucose | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
558 | - |
D-glucose | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
666 | - |
D-glucose | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
686 | - |
D-glucose | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.5 | - |
cellobiose | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
14.5 | - |
cellobiose | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
15.5 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
21.4 | - |
cellobiose | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
22.7 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.25 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
23.3 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.5 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
25.2 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, presence of 0.1 M glucose, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
28.3 | - |
cellobiose | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
28.5 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant W127F, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
36.4 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
39.7 | - |
cellobiose | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
41.6 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant V176A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
45.4 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178E, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
49.4 | - |
cellobiose | mutant C174V, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
70.4 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant H229S, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
78.2 | - |
4-nitrophenyl beta-D-glucopyranoside | mutant L178A, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
79.3 | - |
cellobiose | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens | |
89.9 | - |
4-nitrophenyl beta-D-glucopyranoside | wild-type, pH not specified in the publication, temperature not specified in the publication | Agrobacterium tumefaciens |