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Literature summary for 3.2.1.21 extracted from
- Screening, identification, and characterization of a novel saccharide-stimulated beta-glycosidase from a soil metagenomic library (2017), Appl. Microbiol. Biotechnol., 101, 633-646 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | - |
uncultured microorganism |  |
| D-galactose | - |
uncultured microorganism | |
| D-glucose | - |
uncultured microorganism | |
| D-xylose | - |
uncultured microorganism | |
| maltose | - |
uncultured microorganism | |
Application
| Application | Comment | Organism |
|---|---|---|
| biofuel production | the saccharification yield of rice straw using Trichoderma reesei cellulase is improved by the addition of MeBglD2. These results show that MeBglD2 can be used to improve plant biomass saccharification, because both substrates and products can activate its enzymatic activity | uncultured microorganism |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned into the pET-28b vector. His-tagged MeBglD2 is expressed in Escherichia coli BL21(DE3) cells | uncultured microorganism |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | 5 mM, 49% inhibition | uncultured microorganism | |
| DMSO | 10%, 21% inhibition. 25%, 85% inhibition | uncultured microorganism | |
| EDTA | 10 mM, 10% inhibition | uncultured microorganism | |
| ethanol | 10%, 79% inhibition. 25%, 97% inhibition | uncultured microorganism | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.47 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 0.49 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 0.65 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 0.77 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 0.83 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 0.98 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 1.3 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
| 2.22 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
| 2.43 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
| 3.93 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
| 6.18 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 7.44 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 7.65 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 8.21 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
| 13.3 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CaCl2 | 5 mM, 1.1fold activation | uncultured microorganism | |
| ZnSO4 | 5 mM, 1.26fold activation | uncultured microorganism | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured microorganism | A0A1E1FFN6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
uncultured microorganism |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.68 | - |
substrate: 4-nitrophenyl alpha-D-glucopyranoside, pH 6.0, 60°C | uncultured microorganism |
| 4.15 | - |
substrate: 4-nitrophenyl beta-D-xylopyranoside, pH 6.0, 60°C | uncultured microorganism |
| 25.7 | - |
substrate: 4-nitrophenyl beta-D-glucopyranoside, pH 6.0, 60°C | uncultured microorganism |
| 27.2 | - |
substrate: 4-nitrophenyl beta-D-fucopyranoside, pH 6.0, 60°C | uncultured microorganism |
| 36.2 | - |
substrate: 4-nitrophenyl beta-D-galactopyranoside, pH 6.0, 60°C | uncultured microorganism |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-glucopyranoside + H2O | 7.4% of the activity with 4-nitrophenyl beta-D-galactopyranoside | uncultured microorganism | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-fucopyranoside + H2O | 75% of the activity with 4-nitrophenyl beta-D-galactopyranoside | uncultured microorganism | 4-nitrophenol + beta-D-fucopyranose | - |
? | |
| 4-nitrophenyl beta-D-galactopyranoside + H2O | - |
uncultured microorganism | 4-nitrophenol + beta-D-galactopyranose | - |
? | |
| 4-nitrophenyl beta-D-glucopyranoside + H2O | 71% of the activity with 4-nitrophenyl beta-D-galactopyranoside | uncultured microorganism | 4-nitrophenol + beta-D-glucopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | 11% of the activity with 4-nitrophenyl beta-D-galactopyranoside | uncultured microorganism | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| cellobiose + H2O | - |
uncultured microorganism | 2 beta-D-glucose | - |
? | |
| laminaribiose + H2O | - |
uncultured microorganism | 2 beta-D-glucose | - |
? | |
| sophorose + H2O | - |
uncultured microorganism | alpha-D-glucose + beta-D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MeBglD2 | beta-glucosidase/galactosidase/fucosidase activity, cf. 3.2.1.23 and 3.2.1.38 | uncultured microorganism |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 31.2 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 34.8 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 91.1 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 95.6 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 108 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 117 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 128 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 129 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 142 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 187 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
| 197 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
| 236 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
| 286 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
| 486 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
| 600 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 12.5 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 14 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
| 17.3 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 20.7 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 24 | - |
4-nitrophenyl beta-D-galactopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
| 63.7 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 74.5 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, without additive | uncultured microorganism | |
| 96.8 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
| 118 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 124 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-glucose | uncultured microorganism | |
| 129 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 129 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
| 144 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 25 mM cellobiose | uncultured microorganism | |
| 181 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-galactose | uncultured microorganism | |
| 463 | - |
4-nitrophenyl beta-D-fucopyranoside | pH 6.0, 60°C, additive: 250 mM D-xylose | uncultured microorganism | |
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