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Literature summary for 3.2.1.21 extracted from
- A simple method for beta-glucosidase immobilization and its application in soybean isoflavone glycosides hydrolysis (2018), Biotechnol. Bioprocess Eng., 23, 39-48 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain KM71H | Thermoascus aurantiacus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
4-nitrophenyl beta-D-glucoside | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus |  |
| 0.2 | - |
daidzin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 0.4 | - |
genistin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 0.5 | - |
genistin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 0.6 | - |
glycitin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 0.7 | - |
glycitin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 1 | - |
4-nitrophenyl beta-D-glucoside | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 1.1 | - |
daidzin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | the immobilized enzyme shows about 200% activity at 15% (v/v) | Thermoascus aurantiacus |
| Methanol | the immobilized enzyme shows about 175% activity at 15% (v/v) | Thermoascus aurantiacus |
| additional information | the immobilized enzyme is not influenced by acetonitrile | Thermoascus aurantiacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | - |
- |
- |
| Thermoascus aurantiacus IFO9748 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Thermoascus aurantiacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus | 4-nitrophenol + beta-D-glucose | - |
? | |
| 4-nitrophenyl beta-D-glucoside + H2O | - |
Thermoascus aurantiacus IFO9748 | 4-nitrophenol + beta-D-glucose | - |
? | |
| daidzin + H2O | - |
Thermoascus aurantiacus | daidzein + D-glucose | - |
? | |
| daidzin + H2O | - |
Thermoascus aurantiacus IFO9748 | daidzein + D-glucose | - |
? | |
| genistin + H2O | - |
Thermoascus aurantiacus | genistein + D-glucose | - |
? | |
| genistin + H2O | - |
Thermoascus aurantiacus IFO9748 | genistein + D-glucose | - |
? | |
| glycitin + H2O | - |
Thermoascus aurantiacus | glycitin aglycone + D-glucose | - |
? | |
| glycitin + H2O | - |
Thermoascus aurantiacus IFO9748 | glycitin aglycone + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 140000, SDS-PAGE | Thermoascus aurantiacus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BGL1 | - |
Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
free enzyme | Thermoascus aurantiacus |
| 75 | - |
immobilized enzyme | Thermoascus aurantiacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 85 | after 40 h at 40°C, 50°C, 60°C and 70°C, the enzyme shows about 90%, 25%, 25% and less than 5% activity, respectively. The half-lifes of the free enzyme are 2, 8, and 12 h at 70°C, 60°C, and 50°C. When the temperature increases to 85°C, the enzyme loses its activity in 30 min | Thermoascus aurantiacus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 63.3 | - |
daidzin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 63.9 | - |
glycitin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 72.9 | - |
genistin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 79.8 | - |
daidzin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 83.5 | - |
4-nitrophenyl beta-D-glucoside | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 95.8 | - |
glycitin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 116.6 | - |
4-nitrophenyl beta-D-glucoside | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 171.2 | - |
genistin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Thermoascus aurantiacus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | more than 50% activity between pH 3.0 and 9.0 | Thermoascus aurantiacus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | the enzyme is stable for 24 h at pH 3.0 to 9.0 | Thermoascus aurantiacus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 56.9 | - |
daidzin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 87.5 | - |
4-nitrophenyl beta-D-glucoside | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 95.9 | - |
glycitin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 141.5 | - |
genistin | free enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 142.5 | - |
glycitin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 369.2 | - |
daidzin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 420.4 | - |
genistin | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
| 566.3 | - |
4-nitrophenyl beta-D-glucoside | immobilized enzyme, at pH 5.0 and 37°C | Thermoascus aurantiacus | |
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Release 2023.1 (January 2023)
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