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Literature summary for 3.2.1.22 extracted from

  • Sripuan, T.; Aoki, K.; Yamamoto, K.; Tongkao, D.; Kumagai, H.
    Purification and characterization of thermostable alpha-galactosidase from Ganoderma lucidum (2003), Biosci. Biotechnol. Biochem., 67, 1485-1491.
    View publication on PubMed
Search for more literature for 3.2.1.22

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 68% inhibition Ganoderma lucidum
D-galactose 10 mM, complete inhibition Ganoderma lucidum
D-xylose 10 mM, 12% inhibition Ganoderma lucidum
Hg2+ 1 mM, 91% inhibition Ganoderma lucidum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 p-nitrophenyl alpha-D-galactopyranoside pH 6.0, 40°C Ganoderma lucidum

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ 1 mM, 1.2fold stimulation Ganoderma lucidum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 4 * 56000, SDS-PAGE Ganoderma lucidum
249000
-
 gel filtration Ganoderma lucidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
melibiose + H2O Ganoderma lucidum 42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside alpha-D-galactose + D-glucose
-
 ?
o-nitrophenyl alpha-D-galactopyranoside + H2O Ganoderma lucidum 27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside o-nitrophenol + D-galactose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + D-glucose Ganoderma lucidum transglycosylation ?
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O Ganoderma lucidum
-
 p-nitrophenol + alpha-D-galactose
-
 ?
raffinose + H2O Ganoderma lucidum 52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside sucrose + alpha-D-galactose
-
 ?
stachyose + 2 H2O Ganoderma lucidum 69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside 2 alpha-D-galactose + sucrose
-
 ?

Organism

Organism UniProt Comment Textmining
Ganoderma lucidum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ganoderma lucidum

Source Tissue

Source Tissue Comment Organism Textmining
fruit body fresh Ganoderma lucidum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
23.2
-
-
 Ganoderma lucidum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
melibiose + H2O 42.1% of the activity with p-nitrophenyl alpha-D-galactopyranoside Ganoderma lucidum alpha-D-galactose + D-glucose
-
 ?
o-nitrophenyl alpha-D-galactopyranoside + H2O 27.6% of the activity with p-nitrophenyl alpha-D-galactopyranoside Ganoderma lucidum o-nitrophenol + D-galactose
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + D-glucose transglycosylation Ganoderma lucidum ?
-
 ?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Ganoderma lucidum p-nitrophenol + alpha-D-galactose
-
 ?
raffinose + H2O 52.4% of the activity with p-nitrophenyl alpha-D-galactopyranoside Ganoderma lucidum sucrose + alpha-D-galactose
-
 ?
stachyose + 2 H2O 69.7% of the activity with p-nitrophenyl alpha-D-galactopyranoside Ganoderma lucidum 2 alpha-D-galactose + sucrose
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 56000, SDS-PAGE Ganoderma lucidum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Ganoderma lucidum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 30 min, stable Ganoderma lucidum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Ganoderma lucidum