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Literature summary for 3.2.1.22 extracted from

  • King, M.R.; White, B.A.; Blaschek, H.P.; Chassy, B.M.; Mackie, R.I.; Cann, I.K.O.
    Purification and characterization of a thermostable alpha-galactosidase from Thermoanaerobacterium polysaccharolyticum (2002), J. Agric. Food Chem., 50, 5676-5682.
    View publication on PubMed

Application

Application Comment Organism
nutrition food ingredients containing alpha-1,6-galactoside bond elicit gastrointestinal disturbances in monogastric animals, including humans. Pretreatment of such ingredients with alpha-galactosidase has the potential to alleviate this condition Caldanaerobius polysaccharolyticus

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 10 mM, 19.9% inhibition Caldanaerobius polysaccharolyticus
CdSO4 0.25 mM, 99.9% inhibition Caldanaerobius polysaccharolyticus
CoSO4 0.25 mM, 8.3% inhibition Caldanaerobius polysaccharolyticus
CuCl2 1.0 mM, 30.8% inhibition Caldanaerobius polysaccharolyticus
FeCl3 1.0 mM, 46.2% inhibition Caldanaerobius polysaccharolyticus
FeSO4 1.0 mM, 50.7% inhibition Caldanaerobius polysaccharolyticus
galactose
-
Caldanaerobius polysaccharolyticus
HgCl2 1.0 mM, 88.2% inhibition Caldanaerobius polysaccharolyticus
MgCl2 10 mM, 16.4% inhibition Caldanaerobius polysaccharolyticus
MnCl2 1.0 mM, 18.4% inhibition Caldanaerobius polysaccharolyticus
NEM 10 mM, 12.9% inhibition Caldanaerobius polysaccharolyticus
NiCl2 1.0 mM, 21.7% inhibition Caldanaerobius polysaccharolyticus
p-hydroxymercuribenzoate 0.25 mM, 97.8% inhibition Caldanaerobius polysaccharolyticus
ZnCl2 1.0 mM, 99.3% inhibition Caldanaerobius polysaccharolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29 0.35 p-nitrophenyl alpha-D-galactopyranoside
-
Caldanaerobius polysaccharolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
2 * 80000, SDS-PAGE Caldanaerobius polysaccharolyticus
176000
-
gel filtration Caldanaerobius polysaccharolyticus

Organism

Organism UniProt Comment Textmining
Caldanaerobius polysaccharolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Caldanaerobius polysaccharolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
247.1
-
-
Caldanaerobius polysaccharolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
melibiose + H2O
-
Caldanaerobius polysaccharolyticus alpha-D-galactose + alpha-D-glucose
-
?
additional information activity with soy molasses Caldanaerobius polysaccharolyticus ?
-
?
p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Caldanaerobius polysaccharolyticus p-nitrophenol + alpha-D-galactose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Caldanaerobius polysaccharolyticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
78
-
-
Caldanaerobius polysaccharolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
36.5 h, 12% loss of activity Caldanaerobius polysaccharolyticus
70
-
36.5 h, 16% loss of activity Caldanaerobius polysaccharolyticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Caldanaerobius polysaccharolyticus

pH Range

pH Minimum pH Maximum Comment Organism
5.5 10 pH 5.5: about 60% of maximal activity, pH 10.0: about 45% of maximal activity Caldanaerobius polysaccharolyticus