Application | Comment | Organism |
---|---|---|
nutrition | food ingredients containing alpha-1,6-galactoside bond elicit gastrointestinal disturbances in monogastric animals, including humans. Pretreatment of such ingredients with alpha-galactosidase has the potential to alleviate this condition | Caldanaerobius polysaccharolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 10 mM, 19.9% inhibition | Caldanaerobius polysaccharolyticus | |
CdSO4 | 0.25 mM, 99.9% inhibition | Caldanaerobius polysaccharolyticus | |
CoSO4 | 0.25 mM, 8.3% inhibition | Caldanaerobius polysaccharolyticus | |
CuCl2 | 1.0 mM, 30.8% inhibition | Caldanaerobius polysaccharolyticus | |
FeCl3 | 1.0 mM, 46.2% inhibition | Caldanaerobius polysaccharolyticus | |
FeSO4 | 1.0 mM, 50.7% inhibition | Caldanaerobius polysaccharolyticus | |
galactose | - |
Caldanaerobius polysaccharolyticus | |
HgCl2 | 1.0 mM, 88.2% inhibition | Caldanaerobius polysaccharolyticus | |
MgCl2 | 10 mM, 16.4% inhibition | Caldanaerobius polysaccharolyticus | |
MnCl2 | 1.0 mM, 18.4% inhibition | Caldanaerobius polysaccharolyticus | |
NEM | 10 mM, 12.9% inhibition | Caldanaerobius polysaccharolyticus | |
NiCl2 | 1.0 mM, 21.7% inhibition | Caldanaerobius polysaccharolyticus | |
p-hydroxymercuribenzoate | 0.25 mM, 97.8% inhibition | Caldanaerobius polysaccharolyticus | |
ZnCl2 | 1.0 mM, 99.3% inhibition | Caldanaerobius polysaccharolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | 0.35 | p-nitrophenyl alpha-D-galactopyranoside | - |
Caldanaerobius polysaccharolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
2 * 80000, SDS-PAGE | Caldanaerobius polysaccharolyticus |
176000 | - |
gel filtration | Caldanaerobius polysaccharolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldanaerobius polysaccharolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Caldanaerobius polysaccharolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
247.1 | - |
- |
Caldanaerobius polysaccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
melibiose + H2O | - |
Caldanaerobius polysaccharolyticus | alpha-D-galactose + alpha-D-glucose | - |
? | |
additional information | activity with soy molasses | Caldanaerobius polysaccharolyticus | ? | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Caldanaerobius polysaccharolyticus | p-nitrophenol + alpha-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 80000, SDS-PAGE | Caldanaerobius polysaccharolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
78 | - |
- |
Caldanaerobius polysaccharolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
36.5 h, 12% loss of activity | Caldanaerobius polysaccharolyticus |
70 | - |
36.5 h, 16% loss of activity | Caldanaerobius polysaccharolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Caldanaerobius polysaccharolyticus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | pH 5.5: about 60% of maximal activity, pH 10.0: about 45% of maximal activity | Caldanaerobius polysaccharolyticus |