Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | potent inhibitor | Geobacillus stearothermophilus | |
Cu2+ | potent inhibitor | Geobacillus stearothermophilus | |
D-galactose | - |
Geobacillus stearothermophilus | |
Hg2+ | potent inhibitor | Geobacillus stearothermophilus | |
lactose | - |
Geobacillus stearothermophilus | |
melibiose | - |
Geobacillus stearothermophilus | |
additional information | beta-mercaptoethanol (1.0 mM) and urea (1.0 M) do not inhibit the enzyme activity | Geobacillus stearothermophilus | |
PCMB | potent inhibitor | Geobacillus stearothermophilus | |
stachyose | - |
Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ba2+, Ca2+, Co2+, Fe3+, K+, Mg2+, Mn2+, Na+, Ni2+, Zn2+ (1 mM) and EDTA (10 mM) have no influence on enzyme activity | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79900 | - |
SDS-PAGE | Geobacillus stearothermophilus |
82950 | - |
2 * 82950, gel filtration | Geobacillus stearothermophilus |
165900 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus NCIM-5146 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose CL-4B column chromatography | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.03 | - |
culture filtrate | Geobacillus stearothermophilus |
400 | - |
after 388fold purification | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
melibiose + H2O | - |
Geobacillus stearothermophilus | D-galactose + D-glucose | - |
? | |
melibiose + H2O | - |
Geobacillus stearothermophilus NCIM-5146 | D-galactose + D-glucose | - |
? | |
additional information | no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal | Geobacillus stearothermophilus | ? | - |
? | |
additional information | no detectable activity towards methyl-alpha-D-galactopyranoside, locust bean gum, guar gum, and alpha-D-Gal-(1,4)-D-Gal | Geobacillus stearothermophilus NCIM-5146 | ? | - |
? | |
o-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Geobacillus stearothermophilus | o-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Geobacillus stearothermophilus | p-nitrophenol + alpha-D-galactose | - |
? | |
p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Geobacillus stearothermophilus NCIM-5146 | p-nitrophenol + alpha-D-galactose | - |
? | |
raffinose + H2O | - |
Geobacillus stearothermophilus | sucrose + D-galactose | - |
? | |
raffinose + H2O | - |
Geobacillus stearothermophilus NCIM-5146 | sucrose + D-galactose | - |
? | |
stachyose + 2 H2O | - |
Geobacillus stearothermophilus | 2 alpha-D-galactose + sucrose | - |
? | |
stachyose + 2 H2O | - |
Geobacillus stearothermophilus NCIM-5146 | 2 alpha-D-galactose + sucrose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 82950, gel filtration | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
Alpha-D-galactoside galactohydrolase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life of inactivation at 70°C is 30 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Geobacillus stearothermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | - |
Geobacillus stearothermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
14.5 | - |
lactose | - |
Geobacillus stearothermophilus | |
16.25 | - |
D-galactose | - |
Geobacillus stearothermophilus | |
16.25 | - |
melibiose | - |
Geobacillus stearothermophilus | |
33 | - |
stachyose | - |
Geobacillus stearothermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Geobacillus stearothermophilus | isoelectric focusing | - |
4.9 |