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Literature summary for 3.2.1.22 extracted from

  • Zhao, H.; Lu, L.; Xiao, M.; Wang, Q.; Lu, Y.; Liu, C.; Wang, P.; Kumagai, H.; Yamamoto, K.
    Cloning and characterization of a novel alpha-galactosidase from Bifidobacterium breve 203 capable of synthesizing Gal-alpha-1,4 linkage (2008), FEMS Microbiol. Lett., 285, 278-283.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA 16% increase in activity Bifidobacterium breve
NH4+ 23% increase in activity Bifidobacterium breve

Cloned(Commentary)

Cloned (Comment) Organism
-
Bifidobacterium breve

Inhibitors

Inhibitors Comment Organism Structure
Ag+ strong Bifidobacterium breve
Cu2+ strong Bifidobacterium breve
Hg2+ strong Bifidobacterium breve

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.27
-
4-nitrophenyl-alpha-D-galactopyranoside
-
Bifidobacterium breve
4.3
-
melibiose
-
Bifidobacterium breve

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80500
-
2 * 81500, calculated, 2 * 80500, SDS-PAGE Bifidobacterium breve
81500
-
2 * 81500, calculated, 2 * 80500, SDS-PAGE Bifidobacterium breve
152000
-
PAGE Bifidobacterium breve

Organism

Organism UniProt Comment Textmining
Bifidobacterium breve Q2XQ11
-
-
Bifidobacterium breve 203 Q2XQ11
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl alpha-D-galactopyranoside + H2O
-
Bifidobacterium breve 2-nitrophenol + D-galactopyranose 16.5% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside ?
2-nitrophenyl alpha-D-galactopyranoside + H2O
-
Bifidobacterium breve 203 2-nitrophenol + D-galactopyranose 16.5% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside ?
3-nitrophenyl-alpha-D-galactopyranoside + H2O
-
Bifidobacterium breve 3-nitrophenol + D-galactopyranose 5.6% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside ?
3-nitrophenyl-alpha-D-galactopyranoside + H2O
-
Bifidobacterium breve 203 3-nitrophenol + D-galactopyranose 5.6% of the activity with 4-nitrophenyl-alpha-D-galactopyranoside ?
4-nitrophenyl-alpha-D-galactopyranoside + H2O
-
Bifidobacterium breve 4-nitrophenol + D-galactopyranose
-
?
melibiose + H2O
-
Bifidobacterium breve D-galactose + D-glucose
-
?
melibiose + H2O
-
Bifidobacterium breve 203 D-galactose + D-glucose
-
?
additional information enzyme additionally catalyzes transglycosylation. It is able to synthesize a trisaccharide galactose-(alpha-1,4)-galactose(alpha-1,6)-glucose using melibiose as substrate. In presence of 4-nitrophenyl-alpha-D-galactopyranoside, enzyme catalyzes glycosyl transfer to various acceptors Bifidobacterium breve ?
-
?
additional information no substrate: 3-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galacturonide, 2-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside, and 2-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside Bifidobacterium breve ?
-
?
additional information enzyme additionally catalyzes transglycosylation. It is able to synthesize a trisaccharide galactose-(alpha-1,4)-galactose(alpha-1,6)-glucose using melibiose as substrate. In presence of 4-nitrophenyl-alpha-D-galactopyranoside, enzyme catalyzes glycosyl transfer to various acceptors Bifidobacterium breve 203 ?
-
?
additional information no substrate: 3-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-beta-D-galacturonide, 2-nitrophenyl-alpha-D-glucopyranoside, 2-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-L-fucopyranoside, 4-nitrophenyl-beta-D-fucopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-alpha-D-galactopyranoside, 4-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside, and 2-nitrophenyl-2-(acetylamino)-2-deoxy-beta-D-galactopyranoside Bifidobacterium breve 203 ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 81500, calculated, 2 * 80500, SDS-PAGE Bifidobacterium breve

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Bifidobacterium breve

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable below Bifidobacterium breve

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Bifidobacterium breve

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9
-
Bifidobacterium breve