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Literature summary for 3.2.1.22 extracted from

  • Zhou, J.; Dong, Y.; Li, J.; Zhang, R.; Tang, X.; Mu, Y.; Xu, B.; Wu, Q.; Huang, Z.
    Cloning, heterologous expression, and characterization of novel protease-resistant alpha-galactosidase from new Sphingomonas strain (2012), J. Microbiol. Biotechnol., 22, 1532-1539.
    View publication on PubMed
Search for more literature for 3.2.1.22

Activating Compound

Activating Compound Comment Organism Structure
EDTA 114.7% activity at 10 mM Sphingomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Sphingomonas sp.

General Stability

General Stability Organism
the enzyme shows strong resistance to trypsin and proteinase K digestion. After treatment at 37°C for 1 h with trypsin and proteinase K, the enzyme retained 102.1% and 114% of its initial activity, respectively Sphingomonas sp.

Inhibitors

Inhibitors Comment Organism Structure
Ag+ complete inhibition at 10 mM Sphingomonas sp.
FeSO4 69.5% residual acitivty at 10 mM Sphingomonas sp.
Hg2+ complete inhibition at 10 mM Sphingomonas sp.
Mg2+ 93.4% residual activity at 10 mM Sphingomonas sp.
additional information not inhibited by EDTA, 2-mercaptoethanol Sphingomonas sp.
SDS 7.6% residual acitivty at 10 mM Sphingomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2
-
 4-nitrophenyl alpha-D-galactopyranoside at pH 5.0 and 60°C Sphingomonas sp.
29.4
-
 melibiose at pH 5.0 and 60°C Sphingomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 119.7% activity at 10 mM Sphingomonas sp.
Mn2+ 110.8% activity at 10 mM Sphingomonas sp.
additional information not influenced by Zn2+, Fe3+, K+, and Cu2+ Sphingomonas sp.
Na+ 104.9% activity at 10 mM Sphingomonas sp.
Ni2+ 113.7% activity at 10 mM Sphingomonas sp.
Pb2+ 113.5% activity at 10 mM Sphingomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82300
-
 x * 82300, calculated from amino acid sequence Sphingomonas sp.
85500
-
 x * 85500, SDS-PAGE Sphingomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
melibiose + H2O Sphingomonas sp.
-
 D-galactose + D-glucose
-
 ?
melibiose + H2O Sphingomonas sp. JB13
-
 D-galactose + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingomonas sp. M4GRS1
-
-
Sphingomonas sp. JB13 M4GRS1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Sphingomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 with raffinose as substrate, at pH 5.0 and 60°C Sphingomonas sp.
2.8
-
 with cottonseed meal as substrate, at pH 5.0 and 60°C Sphingomonas sp.
6.9
-
 with soybean meal as substrate, at pH 5.0 and 60°C Sphingomonas sp.
221
-
 with melibiose as substrate, at pH 5.0 and 60°C Sphingomonas sp.
225
-
 with 4-nitrophenyl alpha-D-galactopyranoside as substrate, at pH 5.0 and 60°C Sphingomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-galactopyranoside + H2O best substrate Sphingomonas sp. 4-nitrophenol + alpha-D-galactopyranose
-
 ?
4-nitrophenyl alpha-D-galactopyranoside + H2O best substrate Sphingomonas sp. JB13 4-nitrophenol + alpha-D-galactopyranose
-
 ?
cottonseed meal + H2O
-
 Sphingomonas sp. ?
-
 ?
cottonseed meal + H2O
-
 Sphingomonas sp. JB13 ?
-
 ?
melibiose + H2O
-
 Sphingomonas sp. D-galactose + D-glucose
-
 ?
melibiose + H2O
-
 Sphingomonas sp. JB13 D-galactose + D-glucose
-
 ?
raffinose + H2O worst substrate Sphingomonas sp. sucrose + D-galactose
-
 ?
raffinose + H2O worst substrate Sphingomonas sp. JB13 sucrose + D-galactose
-
 ?
soybean meal + H2O
-
 Sphingomonas sp. ?
-
 ?
soybean meal + H2O
-
 Sphingomonas sp. JB13 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 82300, calculated from amino acid sequence Sphingomonas sp.
? x * 85500, SDS-PAGE Sphingomonas sp.

Synonyms

Synonyms Comment Organism
1,6-alpha-D-galactoside galactohydrolase
-
 Sphingomonas sp.
agaAJB13
-
 Sphingomonas sp.
melibiase
-
 Sphingomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Sphingomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 65 more than 50% activity between 30 and 65°C Sphingomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 the enzyme has a half-life of more than 60 min at 60°C and retains about 25% activity at 20°C, and about 10% at 10°C. At 65°C and 70°C, the enzyme activity decreases rapidly Sphingomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
448
-
 melibiose at pH 5.0 and 60°C Sphingomonas sp.
512
-
 4-nitrophenyl alpha-D-galactopyranoside at pH 5.0 and 60°C Sphingomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Sphingomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
4.5 6.5 more than 50% activity between pH 4.5 and 6.5 Sphingomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 11 the enzyme exhibits more than 40% of its initial activity after incubation in buffers ranging from pH 4.0 to 11.0 at 37°C for 1 h Sphingomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Sphingomonas sp. calculated from amino acid sequence
-
 5.2