Any feedback?
Literature summary for 3.2.1.22 extracted from
- A novel thermostable alpha-galactosidase from the thermophilic fungus Thermomyces lanuginosus CBS 395.62/b purification and characterization (2007), Biochim. Biophys. Acta, 1770, 55-62 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 10 mM, complete inactivation | Thermomyces lanuginosus |  |
| Ca2+ | - |
Thermomyces lanuginosus | |
| Hg2+ | - |
Thermomyces lanuginosus | |
| Zn2+ | - |
Thermomyces lanuginosus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.13 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
| 1.17 | - |
stachyose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
| 1.61 | - |
raffinose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermomyces lanuginosus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | - |
Thermomyces lanuginosus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermomyces lanuginosus | - |
- |
- |
| Thermomyces lanuginosus CBS 395.62/b | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate content 5.3% | Thermomyces lanuginosus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermomyces lanuginosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture fluid | - |
Thermomyces lanuginosus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1813 | - |
pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 4-5 days, 60-70% loss of activity | Thermomyces lanuginosus |
| 4°C, 4-5 days, 60-70% loss of activity | Thermomyces lanuginosus |
| highly stable in freeze-dried form or in buffered solutions containing 10-20% (w/v) NaCl | Thermomyces lanuginosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermomyces lanuginosus | 4-nitrophenol + alpha-D-galactose | - |
? | |
| 4-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermomyces lanuginosus CBS 395.62/b | 4-nitrophenol + alpha-D-galactose | - |
? | |
| raffinose + H2O | - |
Thermomyces lanuginosus | D-galactose + sucrose | - |
? | |
| raffinose + H2O | - |
Thermomyces lanuginosus CBS 395.62/b | D-galactose + sucrose | - |
? | |
| stachyose + H2O | - |
Thermomyces lanuginosus | raffinose + D-galactose | - |
? | |
| stachyose + H2O | - |
Thermomyces lanuginosus CBS 395.62/b | raffinose + D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 93000, SDS-PAGE | Thermomyces lanuginosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Thermomyces lanuginosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
pH 6.4-8.3, stable for at least 24 h | Thermomyces lanuginosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3914 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
| 6946 | - |
raffinose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
| 7659 | - |
stachyose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Thermomyces lanuginosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | 8.3 | 55°C, stable for at least 24 h | Thermomyces lanuginosus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermomyces lanuginosus | isoelectric focusing | - |
3.9 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3455 | - |
4-nitrophenyl alpha-D-galactopyranoside | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
| 4305 | - |
raffinose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
| 6555 | - |
stachyose | pH not specified in the publication, temperature not specified in the publication | Thermomyces lanuginosus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
