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Literature summary for 3.2.1.23 extracted from

  • Itoh, T.; Suzuki, M.; Adachi, S.
    Production and characterization of beta-galactosidase from lactose-fermenting yeasts (1982), Agric. Biol. Chem., 46, 899-904.
No PubMed abstract available
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 0.2 mM, 55-65% inhibition Kluyveromyces marxianus
Ca2+
-
 Torulopsis sphaerica
Ca2+ 0.2 mM, 55-65% inhibition Wickerhamiella versatilis
cellobiose slight inhibition Kluyveromyces marxianus
cellobiose slight inhibition Torulopsis sphaerica
cellobiose slight inhibition Wickerhamiella versatilis
D-galactose slight inhibition Kluyveromyces marxianus
D-galactose slight inhibition Torulopsis sphaerica
D-galactose slight inhibition Wickerhamiella versatilis
D-glucose slight inhibition Kluyveromyces marxianus
D-glucose slight inhibition Torulopsis sphaerica
D-glucose slight inhibition Wickerhamiella versatilis
fructose slight inhibition Kluyveromyces marxianus
fructose slight inhibition Torulopsis sphaerica
fructose slight inhibition Wickerhamiella versatilis
Hg2+ 0.2 mM, complete inhibition Kluyveromyces marxianus
Hg2+ 0.2 mM, complete inhibition Torulopsis sphaerica
Hg2+
-
 Wickerhamiella versatilis
iodoacetamide
-
 Kluyveromyces marxianus
iodoacetamide
-
 Torulopsis sphaerica
iodoacetamide
-
 Wickerhamiella versatilis
maltose slight inhibition Kluyveromyces marxianus
maltose slight inhibition Torulopsis sphaerica
maltose slight inhibition Wickerhamiella versatilis
Mg2+ weak Kluyveromyces marxianus
Mg2+
-
 Torulopsis sphaerica
Mg2+ weak Wickerhamiella versatilis
NEM
-
 Kluyveromyces marxianus
NEM
-
 Torulopsis sphaerica
NEM
-
 Wickerhamiella versatilis
sucrose slight inhibition Kluyveromyces marxianus
sucrose slight inhibition Torulopsis sphaerica
sucrose slight inhibition Wickerhamiella versatilis
xylose slight inhibition Torulopsis sphaerica
xylose slight inhibition Wickerhamiella versatilis
Zn2+ 0.2 mM Kluyveromyces marxianus
Zn2+
-
 Torulopsis sphaerica
Zn2+ 0.2 mM Wickerhamiella versatilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.09
-
 o-nitrophenyl-beta-D-galactoside
-
 Torulopsis sphaerica
2.82
-
 o-nitrophenyl-beta-D-galactoside strain A60 Kluyveromyces marxianus
3.45
-
 o-nitrophenyl-beta-D-galactoside strain B57 Kluyveromyces marxianus
3.45
-
 o-nitrophenyl-beta-D-galactoside strain M6 Wickerhamiella versatilis
16.7
-
 lactose strain A60 Kluyveromyces marxianus
25
-
 lactose
-
 Torulopsis sphaerica

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 0.2 mM, slight activation Kluyveromyces marxianus
Mn2+ 0.2 mM, slight activation Wickerhamiella versatilis
Mn2+ 0.2 mM, slight activation Torulopsis sphaerica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 strain B57, gel filtration Kluyveromyces marxianus
215000
-
 strain A60, gel filtration Kluyveromyces marxianus
226000
-
 gel filtration Wickerhamiella versatilis
233000
-
 gel filtration Torulopsis sphaerica

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus
-
 and strain A60
-
Kluyveromyces marxianus B57
-
 and strain A60
-
Torulopsis sphaerica
-
-
-
Torulopsis sphaerica J28
-
-
-
Wickerhamiella versatilis
-
-
-
Wickerhamiella versatilis M6
-
-
-

Storage Stability

Storage Stability Organism
4°C, lyophilized preparation, stable for 6 months Kluyveromyces marxianus
4°C, lyophilized preparation, stable for 6 months Wickerhamiella versatilis
4°C, lyophilized preparation, stable for 6 months Torulopsis sphaerica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
 Torulopsis sphaerica D-galactose + D-glucose
-
 ?
lactose + H2O
-
 Torulopsis sphaerica J28 D-galactose + D-glucose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Kluyveromyces marxianus o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Wickerhamiella versatilis o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Torulopsis sphaerica o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Torulopsis sphaerica J28 o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Wickerhamiella versatilis M6 o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Kluyveromyces marxianus B57 o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Kluyveromyces marxianus
30
-
-
 Wickerhamiella versatilis
30
-
-
 Torulopsis sphaerica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 10 min, 50% loss of activity Kluyveromyces marxianus
35
-
 10 min, 50% loss of activity Wickerhamiella versatilis
35
-
 10 min, 50% loss of activity Torulopsis sphaerica
45
-
 10 min, complete inactivation Kluyveromyces marxianus
45
-
 10 min, complete inactivation Wickerhamiella versatilis
45
-
 10 min, complete inactivation Torulopsis sphaerica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Wickerhamiella versatilis
7
-
-
 Torulopsis sphaerica
7.5
-
-
 Kluyveromyces marxianus