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Literature summary for 3.2.1.23 extracted from

  • Watanabe, Y.; Kibesaki, Y.; Takenishi, S.; Sakai, K.; Tsujisaka, Y.
    Purification and properties of beta-galactosidase from Penicillium citrinum (1979), Agric. Biol. Chem., 43, 943-950.
No PubMed abstract available
Search for more literature for 3.2.1.23

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Aspergillus oryzae
Cu2+ 1 mM, 59% inhibition Penicillium citrinum
Hg2+
-
 Aspergillus oryzae
Hg2+ 1 mM, complete inhibition Penicillium citrinum
additional information no substrate inhibition and no product inhibition Penicillium citrinum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7
-
 o-nitrophenyl-beta-D-galactoside
-
 Penicillium citrinum
1.7
-
 p-nitrophenyl-beta-D-galactoside
-
 Penicillium citrinum
1.7
-
 phenyl-beta-D-galactoside
-
 Penicillium citrinum
25
-
 lactose
-
 Penicillium citrinum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Penicillium citrinum
100000
-
 gel filtration Penicillium citrinum
110000
-
 equilibrium sedimentation Penicillium citrinum

Organism

Organism UniProt Comment Textmining
Ascospirella lutea
-
-
-
Aspergillus awamori
-
-
-
Aspergillus cellulosae
-
-
-
Aspergillus niger
-
-
-
Aspergillus oryzae
-
-
-
Fusarium oxysporum
-
-
-
Geotrichum candidum
-
-
-
Mucor javanicus
-
-
-
Mucor mucedo
-
-
-
Neurospora crassa
-
-
-
Penicillium chrysogenum
-
-
-
Penicillium citrinum
-
-
-
Penicillium cyclopium
-
-
-
Penicillium glabrum
-
-
-
Penicillium glaucum
-
-
-
Penicillium roqueforti
-
-
-
Penicillium toxidarium
-
-
-
Rhizopus acidus
-
-
-
Rhizopus arrhizus
-
-
-
Rhizopus formosciensis
-
-
-
Rhizopus microsporus var. chinensis
-
-
-
Rhizopus niveus
-
-
-
Rhizopus stolonifer
-
-
-
Trichoderma viride
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Neurospora crassa
glycoprotein
-
 Aspergillus niger
glycoprotein contains 5.8% carbohydrate Penicillium citrinum

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium citrinum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
98.1
-
-
 Penicillium citrinum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lactose + H2O
-
 Aspergillus niger D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Aspergillus oryzae D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Penicillium citrinum D-glucose + D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Neurospora crassa o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus niger o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus oryzae o-nitrophenol + beta-D-galactose
-
 ?
o-nitrophenyl-beta-D-galactoside + H2O
-
 Penicillium citrinum o-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Penicillium citrinum p-nitrophenol + beta-D-galactose
-
 ?
phenyl-beta-D-galactoside + H2O
-
 Aspergillus oryzae phenol + beta-D-galactose
-
 ?
phenyl-beta-D-galactoside + H2O
-
 Penicillium citrinum phenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE Penicillium citrinum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 pH 4.5, 15 min Penicillium citrinum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
-
 Aspergillus oryzae
40
-
 pH 5.6, 15 min, stable below Penicillium citrinum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Neurospora crassa
4.5
-
-
 Aspergillus oryzae
4.5
-
 at 40°C, 15 min Penicillium citrinum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 7 40°C, 3 h, stable Penicillium citrinum