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Literature summary for 3.2.1.23 extracted from
- Purification and properties of a novel thermostable galactooligosaccharide-producing beta-galactosidase from Sterigmatomyces elviae CBS8119 (1995), Appl. Environ. Microbiol., 61, 4026-4030.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Sterigmatomyces elviae |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
lactose | - |
Sterigmatomyces elviae | |
| 9.5 | - |
o-nitrophenyl-beta-D-galactoside | - |
Sterigmatomyces elviae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 86000 | - |
2 * 86000, SDS-PAGE | Sterigmatomyces elviae |
| 170000 | - |
gel filtration | Sterigmatomyces elviae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sterigmatomyces elviae | - |
- |
- |
| Sterigmatomyces elviae CBS8119 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sterigmatomyces elviae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 20.2 | - |
- |
Sterigmatomyces elviae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Sterigmatomyces elviae | D-glucose + D-galactose | - |
? | |
| lactose + H2O | - |
Sterigmatomyces elviae CBS8119 | D-glucose + D-galactose | - |
? | |
| additional information | high level of transgalactosylation activity | Sterigmatomyces elviae | ? | - |
? | |
| additional information | high level of transgalactosylation activity | Sterigmatomyces elviae CBS8119 | ? | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Sterigmatomyces elviae | o-nitrophenol + beta-D-galactose | - |
? | |
| o-nitrophenyl-beta-D-galactoside + H2O | - |
Sterigmatomyces elviae CBS8119 | o-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 86000, SDS-PAGE | Sterigmatomyces elviae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Sterigmatomyces elviae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
1 h, stable | Sterigmatomyces elviae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5 | - |
Sterigmatomyces elviae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 7 | stable | Sterigmatomyces elviae |
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