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Literature summary for 3.2.1.23 extracted from

  • Nakao, M.; Harada, M.; Kodama, Y.; Nakayama, T.; Shibano, Y.; Amachi, T.
    Purification and characterization of a thermostable beta-galactosidase with high transgalactosylation activity from Saccharopolyspora rectivirgula (1994), Appl. Microbiol. Biotechnol., 40, 657-663.
No PubMed abstract available
Search for more literature for 3.2.1.23

Application

Application Comment Organism
nutrition enzyme potentially serves as an inexpensive beta-galactosidase in food industry Saccharopolyspora rectivirgula

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Saccharopolyspora rectivirgula
Cu2+
-
 Saccharopolyspora rectivirgula
D-galactosamine
-
 Saccharopolyspora rectivirgula
D-galactose
-
 Saccharopolyspora rectivirgula
EDTA
-
 Saccharopolyspora rectivirgula
EGTA
-
 Saccharopolyspora rectivirgula
isopropyl beta-D-thiogalactoside
-
 Saccharopolyspora rectivirgula
methyl beta-D-thiogalactoside
-
 Saccharopolyspora rectivirgula
Pb2+
-
 Saccharopolyspora rectivirgula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 p-nitrophenyl-beta-D-galactoside
-
 Saccharopolyspora rectivirgula
0.75
-
 lactose
-
 Saccharopolyspora rectivirgula

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Saccharopolyspora rectivirgula
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 0.01 mM, activates Saccharopolyspora rectivirgula
K+ 10 mM, activates Saccharopolyspora rectivirgula
Mg2+ 0.01 mM, activates Saccharopolyspora rectivirgula
Mn2+ 0.01 mM, activates Saccharopolyspora rectivirgula
Na+ 10 mM, activates Saccharopolyspora rectivirgula
Na+ half-maximal activation at 0.1 mM Saccharopolyspora rectivirgula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
145000
-
 determination of sedimentation velocity and sedimention equilibrium Saccharopolyspora rectivirgula

Organism

Organism UniProt Comment Textmining
Saccharopolyspora rectivirgula
-
-
-
Saccharopolyspora rectivirgula V2-2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharopolyspora rectivirgula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharopolyspora rectivirgula 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharopolyspora rectivirgula V2-2 2-nitrophenol + beta-D-galactose
-
 ?
lactose transgalactosylation Saccharopolyspora rectivirgula additional information oligosaccharides with the general structure (Gal)n-Glc, where n is 1, 2, 3, and 4 with a glucose at a reducing terminal ?
lactose transgalactosylation Saccharopolyspora rectivirgula V2-2 additional information oligosaccharides with the general structure (Gal)n-Glc, where n is 1, 2, 3, and 4 with a glucose at a reducing terminal ?
lactose + H2O
-
 Saccharopolyspora rectivirgula D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Saccharopolyspora rectivirgula V2-2 D-glucose + D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Saccharopolyspora rectivirgula p-nitrophenol + beta-D-galactose
-
 ?
p-nitrophenyl-beta-D-galactoside + H2O
-
 Saccharopolyspora rectivirgula V2-2 p-nitrophenol + beta-D-galactose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 145000, SDS-PAGE Saccharopolyspora rectivirgula

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 pH 7.2, 4 h, in presence of 1.75 M lactose and 0.01 mM MnCl2, stable up to Saccharopolyspora rectivirgula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47.6
-
 o-nitrophenyl-beta-D-galactoside
-
 Saccharopolyspora rectivirgula
55.8
-
 p-nitrophenyl-beta-D-galactoside
-
 Saccharopolyspora rectivirgula
63.1
-
 lactose
-
 Saccharopolyspora rectivirgula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.2 hydrolysis of lactose and p-nitrophenyl-beta-D-galactoside Saccharopolyspora rectivirgula