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Literature summary for 3.2.1.23 extracted from
- Expression and characterization of Kluyveromyces lactis beta-galactosidase in Escherichia coli (2003), Biotechnol. Lett., 25, 1769-1774.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as a soluble His-tagged recombinant enzyme | Kluyveromyces lactis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | 1 mM, 56% inhibition of activity with o-nitrophenyl beta-D-galactopyranoside | Kluyveromyces lactis |  |
| Ca2+ | 1 mM, 60% inhibition of activity with lactose | Kluyveromyces lactis | |
| Cu2+ | 1 mM, 97% inhibition of activity with o-nitrophenyl beta-D-galactopyranoside, 88% inhibition of activity with lactose | Kluyveromyces lactis | |
| imidazole | above 50 mM, irreversible | Kluyveromyces lactis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.5 | - |
o-nitrophenyl beta-D-galactopyranoside | pH 7.0, 37°C | Kluyveromyces lactis | |
| 19.8 | - |
lactose | pH 7.0, 37°C | Kluyveromyces lactis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 1.85fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside | Kluyveromyces lactis | |
| Co2+ | 1 mM, 8.1fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside, 4.7fold stimulation of activity with lactose | Kluyveromyces lactis | |
| Fe2+ | 1 mM, 4.5fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside, 4.1fold stimulation of activity with lactose | Kluyveromyces lactis | |
| Mg2+ | 1 mM, 7fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside, 5.4fold stimulation of activity with lactose | Kluyveromyces lactis | |
| Mn2+ | 1 mM, 8.9fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside, 5.6fold stimulation of activity with lactose | Kluyveromyces lactis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces lactis | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lactose + H2O | - |
Kluyveromyces lactis | D-glucose + D-galactose | - |
? | |
| o-nitrophenyl beta-D-galactopyranoside + H2O | - |
Kluyveromyces lactis | o-nitrophenol + beta-D-galactose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
hydrolysis of o-nitrophenyl beta-D-galactopyranoside | Kluyveromyces lactis |
| 40 | - |
hydrolysis of lactose | Kluyveromyces lactis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
hydrolysis of o-nitrophenyl beta-D-galactopyranoside or lactose | Kluyveromyces lactis |
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Release 2023.1 (January 2023)
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