Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
according to its CD spectrum, beta-galactosidase is mainly a beta-type protein. The antiparallel beta-sheet appears as the amino type of regular conformation (25%) | Kluyveromyces lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Kluyveromyces lactis | - |
D-glucose + D-galactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Kluyveromyces lactis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Kluyveromyces lactis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1709 | - |
purified commercial preparation | Kluyveromyces lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Kluyveromyces lactis | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Kluyveromyces lactis | D-glucose + D-galactose | - |
? | |
additional information | structure-activity relationship as a function of the pH, overview | Kluyveromyces lactis | ? | - |
? | |
o-nitrophenyl-beta-D-galactoside + H2O | - |
Kluyveromyces lactis | o-nitrophenol + beta-D-galactose | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | secondary structure by circular dichroism, the enzyme is a beta-type protein, having 22% beta-turns, 14% parallel beta-sheet, 25% antiparallel beta-sheet,34% unordered structure, and only 5% alpha-helix, structure-function relationship, highest secondary structure content at pH 6.5-7.0, overview | Kluyveromyces lactis |
Synonyms | Comment | Organism |
---|---|---|
lactase | - |
Kluyveromyces lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Kluyveromyces lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
structure-activity relationship as a function of the pH, overview | Kluyveromyces lactis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Kluyveromyces lactis |