Application | Comment | Organism |
---|---|---|
medicine | use of enzyme as a digestive supplement | Kluyveromyces marxianus |
nutrition | the enzyme has a potential application as a digestive supplement | Kluyveromyces marxianus |
nutrition | use of enzyme as a digestive supplement | Kluyveromyces marxianus |
General Stability | Organism |
---|---|
the unpurified beta-galactosidase displays a high level of stability when exposed to simulated intestinal conditions in vitro for 4 h | Kluyveromyces marxianus |
the unpurified enzyme displays a high level of stability when exposed to simulated intestinal conditions in vitro for 4 h | Kluyveromyces marxianus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactose | 5 mM, 93% residual activity | Kluyveromyces marxianus | |
D-glucose | 5 mM, 86% residual activity | Kluyveromyces marxianus | |
isopropyl beta-D-thiogalactoside | 5 mM, 91% residual activity | Kluyveromyces marxianus | |
additional information | no or poor inhibition by D-glucose, D-galactose, IPTG, and sucrose; no or poor inhibition by D-glucose, D-galactose, IPTG, and sucrose | Kluyveromyces marxianus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4 | - |
lactose | pH 6.8 | Kluyveromyces marxianus | |
4 | - |
lactose | pH 6.8, 37°C | Kluyveromyces marxianus | |
4.1 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 6.8, 37°C | Kluyveromyces marxianus | |
4.7 | - |
2-nitrophenyl-beta-D-galactoside | pH 6.8 | Kluyveromyces marxianus | |
7.1 | - |
4-nitrophenyl-beta-D-galactoside | pH 6.8 | Kluyveromyces marxianus | |
7.1 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 6.8, 37°C | Kluyveromyces marxianus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
123000 | - |
2 * 123000, SDS-PAGE | Kluyveromyces marxianus |
251000 | - |
gel filtration | Kluyveromyces marxianus |
251000 | - |
PAGE | Kluyveromyces marxianus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Kluyveromyces marxianus | the enzyme is involved in lactose hydrolysis in the small intestine of the host organism | D-glucose + D-galactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces marxianus | - |
strain DSM5418 | - |
Kluyveromyces marxianus | P00723 | strain DSM 5418 | - |
Kluyveromyces marxianus | Q6QTF4 | strain DSM 5418 | - |
Kluyveromyces marxianus DSM 5418 | - |
strain DSM5418 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | - |
Kluyveromyces marxianus |
proteolytic modification | pancreatin activates the beta-galactosidase due to a partial proteolytic cleavage yielding a more active enzyme form | Kluyveromyces marxianus |
Purification (Comment) | Organism |
---|---|
- |
Kluyveromyces marxianus |
native enzyme 115fold to homogeneity by gel filtration, and anion exchange and hydroxylapatite chromatography | Kluyveromyces marxianus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Kluyveromyces marxianus |
790 | - |
pH 6.8 | Kluyveromyces marxianus |
790.6 | - |
purified enzyme | Kluyveromyces marxianus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Kluyveromyces marxianus | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactoside + H2O | - |
Kluyveromyces marxianus | 2-nitrophenol + D-galactose | - |
? | |
2-nitrophenyl beta-D-galactoside + H2O | - |
Kluyveromyces marxianus DSM 5418 | 2-nitrophenol + D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Kluyveromyces marxianus | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Kluyveromyces marxianus | 4-nitrophenol + D-galactose | - |
? | |
4-nitrophenyl beta-D-galactoside + H2O | - |
Kluyveromyces marxianus DSM 5418 | 4-nitrophenol + D-galactose | - |
? | |
lactose + H2O | - |
Kluyveromyces marxianus | D-glucose + D-galactose | - |
? | |
lactose + H2O | the enzyme is involved in lactose hydrolysis in the small intestine of the host organism | Kluyveromyces marxianus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Kluyveromyces marxianus | D-galactose + D-glucose | - |
? | |
lactose + H2O | - |
Kluyveromyces marxianus DSM 5418 | D-galactose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 123000, SDS-PAGE | Kluyveromyces marxianus |
More | peptide mass fingerprinting | Kluyveromyces marxianus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Kluyveromyces marxianus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | 50 | temperature profile, about 50% of maximal activity between 20°C and 45°C | Kluyveromyces marxianus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6300 | - |
lactose | pH 6.8 | Kluyveromyces marxianus | |
6300 | - |
lactose | pH 6.8, 37°C | Kluyveromyces marxianus | |
8559 | - |
2-nitrophenyl-beta-D-galactoside | pH 6.8 | Kluyveromyces marxianus | |
8559 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 6.8, 37°C | Kluyveromyces marxianus | |
270000 | - |
4-nitrophenyl-beta-D-galactopyranoside | pH 6.8, 37°C | Kluyveromyces marxianus | |
2700000 | - |
4-nitrophenyl-beta-D-galactoside | pH 6.8 | Kluyveromyces marxianus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Kluyveromyces marxianus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 10 | about 50% of maximal activity between pH 5.6 and pH 7.8 | Kluyveromyces marxianus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Kluyveromyces marxianus | isoelectric focusing | - |
5.1 |