Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose | slightly stimulating | Priestia megaterium | |
D-glucose | slightly stimulating | Priestia megaterium | |
maltose | slightly stimulating | Priestia megaterium | |
additional information | no activation by melibiose and raffinose | Priestia megaterium | |
sucrose | slightly stimulating | Priestia megaterium |
General Stability | Organism |
---|---|
non-specific enzyme stabilization by amino acids, overview | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cs+ | stimulates | Priestia megaterium | |
K+ | stimulates | Priestia megaterium | |
Li+ | stimulates | Priestia megaterium | |
Mn2+ | stimulates | Priestia megaterium | |
Na+ | stimulates | Priestia megaterium | |
NH4+ | stimulates | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
inducible enzyme | - |
Priestia megaterium KM / ATCC 13632 | - |
inducible enzyme | - |
Purification (Comment) | Organism |
---|---|
native enzyme by MnSO4 or streptomycin precipitation, ammonium sulfate fractionation, and starch column electrophoresis | Priestia megaterium |
Storage Stability | Organism |
---|---|
-20°C, crude enzyme extract, 24 h, fully stable, loss of 49% activity after 12 days, partially purified enzyme is stable for 60 days | Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Priestia megaterium | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Priestia megaterium KM / ATCC 13632 | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Priestia megaterium | beta-D-glucose + beta-D-galactose | - |
? | |
lactose + H2O | - |
Priestia megaterium KM / ATCC 13632 | beta-D-glucose + beta-D-galactose | - |
? | |
additional information | the enzyme hydrolyzes methyl-, butyl-, and phenyl-beta-D-galactosides, no activity with thiomethyl-and thiophenyl-beta-D-galactosides or with methyl-beta-L-arabinoside | Priestia megaterium | ? | - |
? | |
additional information | the enzyme hydrolyzes methyl-, butyl-, and phenyl-beta-D-galactosides, no activity with thiomethyl-and thiophenyl-beta-D-galactosides or with methyl-beta-L-arabinoside | Priestia megaterium KM / ATCC 13632 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min, loss of 42% activity in 0.5 M K2HPO4 buffer, no activity loss in 1.5 M K2HP04 | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8 | - |
Priestia megaterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH profile | Priestia megaterium |