Cloned (Comment) | Organism |
---|---|
gene bglA, overexpression of His-tagged cold-active beta-galactosidase in Escherichia coli strain DH5alpha | Arthrobacter psychrolactophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 8.0, 10°C, recombinant enzyme | Arthrobacter psychrolactophilus | |
42.1 | - |
lactose | pH 8.0, 10°C, recombinant enzyme | Arthrobacter psychrolactophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Arthrobacter psychrolactophilus | the enzyme is involved in lactose degradation in milk | D-glucose + D-galactose | - |
? | |
lactose + H2O | Arthrobacter psychrolactophilus F2 | the enzyme is involved in lactose degradation in milk | D-glucose + D-galactose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter psychrolactophilus | Q08KN3 | cold-active beta-galactosidase, gene bglA | - |
Arthrobacter psychrolactophilus F2 | Q08KN3 | cold-active beta-galactosidase, gene bglA | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged cold-active enzyme 47.4fold from Escherichia coli by nickel affinity chromatography | Arthrobacter psychrolactophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.4 | - |
purified recombinant His-tagged enzyme | Arthrobacter psychrolactophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Arthrobacter psychrolactophilus | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Arthrobacter psychrolactophilus F2 | 2-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Arthrobacter psychrolactophilus | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-galactopyranoside + H2O | - |
Arthrobacter psychrolactophilus F2 | 4-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Arthrobacter psychrolactophilus | D-glucose + D-galactose | - |
? | |
lactose + H2O | the enzyme is involved in lactose degradation in milk | Arthrobacter psychrolactophilus | D-glucose + D-galactose | - |
? | |
lactose + H2O | - |
Arthrobacter psychrolactophilus F2 | D-glucose + D-galactose | - |
? | |
lactose + H2O | the enzyme is involved in lactose degradation in milk | Arthrobacter psychrolactophilus F2 | D-glucose + D-galactose | - |
? | |
additional information | no activity with other nitrophenyl derivatives, the cold-active enzyme also shows transglycosylation activity forming trisaccharides from acceptor lactose, overview | Arthrobacter psychrolactophilus | ? | - |
? | |
additional information | no activity with other nitrophenyl derivatives, the cold-active enzyme also shows transglycosylation activity forming trisaccharides from acceptor lactose, overview | Arthrobacter psychrolactophilus F2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BglAp | - |
Arthrobacter psychrolactophilus |
cold-active beta-galactosidase | - |
Arthrobacter psychrolactophilus |
More | the enzyme belongs to the glycosyl hydrolase family 2 | Arthrobacter psychrolactophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
- |
Arthrobacter psychrolactophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature activity profile of the recombinant enzyme, overview | Arthrobacter psychrolactophilus |
- |
10 | high activity at 0°C | Arthrobacter psychrolactophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
temperature stability profile of the recombinant enzyme, overview | Arthrobacter psychrolactophilus |
30 | - |
60 min, purified recombinant enzyme, stable up to | Arthrobacter psychrolactophilus |
50 | - |
5 min, rapid inactivation, purified recombinant enzyme | Arthrobacter psychrolactophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.02 | - |
lactose | pH 8.0, 10°C, recombinant enzyme | Arthrobacter psychrolactophilus | |
12.7 | - |
2-nitrophenyl-beta-D-galactopyranoside | pH 8.0, 10°C, recombinant enzyme | Arthrobacter psychrolactophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arthrobacter psychrolactophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH activity profile of the recombinant enzyme, overview | Arthrobacter psychrolactophilus |
7 | 9 | over 80% of maximal activity within this range | Arthrobacter psychrolactophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH stability profile of the recombinant enzyme, overview | Arthrobacter psychrolactophilus |
6 | 10 | purified recombinant enzyme, stable | Arthrobacter psychrolactophilus |