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Literature summary for 3.2.1.23 extracted from

  • Nakagawa, T.; Ikehata, R.; Myoda, T.; Miyaji, T.; Tomizuka, N.
    Overexpression and functional analysis of cold-active beta-galactosidase from Arthrobacter psychrolactophilus strain F2 (2007), Protein Expr. Purif., 54, 295-299.
    View publication on PubMed
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
gene bglA, overexpression of His-tagged cold-active beta-galactosidase in Escherichia coli strain DH5alpha Arthrobacter psychrolactophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8.0, 10°C, recombinant enzyme Arthrobacter psychrolactophilus
42.1
-
 lactose pH 8.0, 10°C, recombinant enzyme Arthrobacter psychrolactophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lactose + H2O Arthrobacter psychrolactophilus the enzyme is involved in lactose degradation in milk D-glucose + D-galactose
-
 ?
lactose + H2O Arthrobacter psychrolactophilus F2 the enzyme is involved in lactose degradation in milk D-glucose + D-galactose
-
 ?

Organism

Organism UniProt Comment Textmining
Arthrobacter psychrolactophilus Q08KN3 cold-active beta-galactosidase, gene bglA
-
Arthrobacter psychrolactophilus F2 Q08KN3 cold-active beta-galactosidase, gene bglA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged cold-active enzyme 47.4fold from Escherichia coli by nickel affinity chromatography Arthrobacter psychrolactophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20.4
-
 purified recombinant His-tagged enzyme Arthrobacter psychrolactophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Arthrobacter psychrolactophilus 2-nitrophenol + beta-D-galactose
-
 ?
2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Arthrobacter psychrolactophilus F2 2-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Arthrobacter psychrolactophilus 4-nitrophenol + beta-D-galactose
-
 ?
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Arthrobacter psychrolactophilus F2 4-nitrophenol + beta-D-galactose
-
 ?
lactose + H2O
-
 Arthrobacter psychrolactophilus D-glucose + D-galactose
-
 ?
lactose + H2O the enzyme is involved in lactose degradation in milk Arthrobacter psychrolactophilus D-glucose + D-galactose
-
 ?
lactose + H2O
-
 Arthrobacter psychrolactophilus F2 D-glucose + D-galactose
-
 ?
lactose + H2O the enzyme is involved in lactose degradation in milk Arthrobacter psychrolactophilus F2 D-glucose + D-galactose
-
 ?
additional information no activity with other nitrophenyl derivatives, the cold-active enzyme also shows transglycosylation activity forming trisaccharides from acceptor lactose, overview Arthrobacter psychrolactophilus ?
-
 ?
additional information no activity with other nitrophenyl derivatives, the cold-active enzyme also shows transglycosylation activity forming trisaccharides from acceptor lactose, overview Arthrobacter psychrolactophilus F2 ?
-
 ?

Synonyms

Synonyms Comment Organism
BglAp
-
 Arthrobacter psychrolactophilus
cold-active beta-galactosidase
-
 Arthrobacter psychrolactophilus
More the enzyme belongs to the glycosyl hydrolase family 2 Arthrobacter psychrolactophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
10
-
-
 Arthrobacter psychrolactophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
 temperature activity profile of the recombinant enzyme, overview Arthrobacter psychrolactophilus
-
 10 high activity at 0°C Arthrobacter psychrolactophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 temperature stability profile of the recombinant enzyme, overview Arthrobacter psychrolactophilus
30
-
 60 min, purified recombinant enzyme, stable up to Arthrobacter psychrolactophilus
50
-
 5 min, rapid inactivation, purified recombinant enzyme Arthrobacter psychrolactophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.02
-
 lactose pH 8.0, 10°C, recombinant enzyme Arthrobacter psychrolactophilus
12.7
-
 2-nitrophenyl-beta-D-galactopyranoside pH 8.0, 10°C, recombinant enzyme Arthrobacter psychrolactophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arthrobacter psychrolactophilus

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
 pH activity profile of the recombinant enzyme, overview Arthrobacter psychrolactophilus
7 9 over 80% of maximal activity within this range Arthrobacter psychrolactophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 pH stability profile of the recombinant enzyme, overview Arthrobacter psychrolactophilus
6 10 purified recombinant enzyme, stable Arthrobacter psychrolactophilus