Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.23 extracted from

  • Terra, V.S.; Homer, K.A.; Rao, S.G.; Andrew, P.W.; Yesilkaya, H.
    Characterization of novel beta-galactosidase activity that contributes to glycoprotein degradation and virulence in Streptococcus pneumoniae (2010), Infect. Immun., 78, 348-357.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptococcus pneumoniae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.91
-
 4-nitrophenyl-beta-D-galactopyranoside at pH 6.5 Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69000
-
 SDS-PAGE Streptococcus pneumoniae

Organism

Organism UniProt Comment Textmining
Streptococcus pneumoniae
-
 serotype 2 strain D39
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Streptococcus pneumoniae

Purification (Commentary)

Purification (Comment) Organism
Imac resin column chromatography Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Streptococcus pneumoniae 4-nitrophenol + beta-D-galactose
-
 ?
additional information the SPD0065 protein shows no activity towards 4-methylumbelliferyl-N-acetyl-alpha-D-glucosaminide, 4-methylumbeliferyl-alpha-D-glucopyranoside, 2-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-D-glucoside 6-phosphate, 4-nitrophenyl-alpha-D-mannose 6-phosphate, 4-nitrophenyl-alpha-D-galactose 6-phosphate, and 4-nitrophenyl-beta-D-glucose 6-phosphate Streptococcus pneumoniae ?
-
 ?

Synonyms

Synonyms Comment Organism
SPD_0065 protein
-
 Streptococcus pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Streptococcus pneumoniae

Expression

Organism Comment Expression
Streptococcus pneumoniae the expression of SPD_0065 protein in cells grown in mucin-containing medium or tissues harvested from infected animals is significantly upregulated compared to that in pneumococci from glucose-containing medium up