Crystallization (Comment) | Organism |
---|---|
mutant enzymes E416Q and E416V | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E416Q | the mutant enzyme does not have a Mg2+ bound at the active site even at high Mg2+ concentrations (200 mM), low catalytic activity and the pH profile is very different from that of the native enzyme | Escherichia coli |
E416V | the mutant enzyme does not have a Mg2+ bound at the active site even at high Mg2+ concentrations (200 mM), low catalytic activity and the pH profile is very different from that of the native enzyme | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galactonolactone | - |
Escherichia coli | |
D-galactose | - |
Escherichia coli | |
lactose | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
2-nitrophenyl-beta-D-galactopyranoside | native enzyme | Escherichia coli | |
9.5 | - |
2-nitrophenyl-beta-D-galactopyranoside | mutant enzyme E416V | Escherichia coli | |
18 | - |
2-nitrophenyl-beta-D-galactopyranoside | mutant enzyme E416Q | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | beta-galactosidase requires Mg2+ for full catalytic activity, one Mg2+ is at each active site but 3-4 others bind to other parts of each subunit | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose + H2O | Escherichia coli | - |
D-galactose + D-glucose | - |
? | |
lactose + H2O | Escherichia coli LMG194 | - |
D-galactose + D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P00722 | - |
- |
Escherichia coli LMG194 | P00722 | - |
- |
Purification (Comment) | Organism |
---|---|
ProBond Ni column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Escherichia coli | 2-nitrophenol + beta-D-galactose | - |
? | |
2-nitrophenyl beta-D-galactopyranoside + H2O | - |
Escherichia coli LMG194 | 2-nitrophenol + beta-D-galactose | - |
? | |
lactose + H2O | - |
Escherichia coli | D-galactose + D-glucose | - |
? | |
lactose + H2O | - |
Escherichia coli LMG194 | D-galactose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-gal | - |
Escherichia coli |
BGA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
54 | - |
2-nitrophenyl-beta-D-galactopyranoside | mutant enzyme E416Q | Escherichia coli | |
81 | - |
2-nitrophenyl-beta-D-galactopyranoside | mutant enzyme E416V | Escherichia coli | |
620 | - |
2-nitrophenyl-beta-D-galactopyranoside | native enzyme | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
D-galactonolactone | native enzyme | Escherichia coli | |
1 | - |
lactose | native enzyme | Escherichia coli | |
4.9 | - |
D-galactonolactone | mutant enzyme E416V | Escherichia coli | |
24 | - |
D-galactose | native enzyme | Escherichia coli | |
24 | - |
D-galactonolactone | mutant enzyme E416Q | Escherichia coli | |
107 | - |
lactose | mutant enzyme E416V | Escherichia coli | |
154 | - |
lactose | mutant enzyme E416Q | Escherichia coli | |
211 | - |
D-galactose | mutant enzyme E416V | Escherichia coli | |
220 | - |
D-galactose | mutant enzyme E416Q | Escherichia coli |