Application | Comment | Organism |
---|---|---|
analysis | enzyme functions both as a marker, promoting colony color development in the presence of a lactose analogue S-gal, and as a reporter enabling quantitative measurement by a simple colorimetric assay. The combination of BgaB expressed from promoters of varying strength with S-gal produces distinct black colonies in aerobic and anaerobic conditions at temperatures ranging from 37 to 60°C, being an advantage over the conventional beta-galactosidase (LacZ) and substrate X-gal. The system allows for construction and measurement of expression levels of a library in just 4 days | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
BgaB | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Geobacillus stearothermophilus |