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Literature summary for 3.2.1.23 extracted from

  • Jensen, T.; Pogrebnyakov, I.; Falkenberg, K.; Redl, S.; Nielsen, A.
    Application of the thermostable beta-galactosidase, BgaB, from Geobacillus stearothermophilus as a versatile reporter under anaerobic and aerobic conditions (2017), AMB Express, 7, 169 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis enzyme functions both as a marker, promoting colony color development in the presence of a lactose analogue S-gal, and as a reporter enabling quantitative measurement by a simple colorimetric assay. The combination of BgaB expressed from promoters of varying strength with S-gal produces distinct black colonies in aerobic and anaerobic conditions at temperatures ranging from 37 to 60°C, being an advantage over the conventional beta-galactosidase (LacZ) and substrate X-gal. The system allows for construction and measurement of expression levels of a library in just 4 days Geobacillus stearothermophilus

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
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-
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Synonyms

Synonyms Comment Organism
BgaB
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Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
Geobacillus stearothermophilus