Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-D-mannopyranose | 55% inhibition at 30 mM | Aspergillus sp. | |
Co2+ | 46% inhibition at 1 mM | Aspergillus sp. | |
Cu2+ | complete inhibition at 1 mM | Aspergillus sp. | |
D-glucose | 67% inhibition at 30 mM | Aspergillus sp. | |
Fe2+ | 54% inhibition at 1 mM | Aspergillus sp. | |
Hg2+ | complete inhibition at 1 mM | Aspergillus sp. | |
Mn2+ | slight inhibition at 1 mM | Aspergillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Aspergillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69200 | - |
6 * 69200, SDS-PAGE | Aspergillus sp. |
412000 | - |
gel filtration | Aspergillus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-(1-2)-mannobiose + H2O | Aspergillus sp. | - |
alpha-D-mannopyranose + D-mannopyranose | - |
? | |
alpha-(1-3)-mannobiose + H2O | Aspergillus sp. | highest activity and affinity | alpha-D-mannopyranose + D-mannopyranose | - |
? | |
alpha-(1-6)-mannobiose | Aspergillus sp. | low activity | alpha-D-mannopyranose + D-mannopyranose | - |
? | |
Man6-glycopeptide + H2O | Aspergillus sp. | - |
alpha-D-mannopyranose + ? | - |
? | |
Man9-glycopeptide + H2O | Aspergillus sp. | - |
alpha-D-mannopyranose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sp. | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 3.8% neutral sugar content | Aspergillus sp. |
Purification (Comment) | Organism |
---|---|
to homogeneity, preparative PAGE | Aspergillus sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Aspergillus sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.36 | - |
- |
Aspergillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-(1-2)-mannobiose + H2O | - |
Aspergillus sp. | alpha-D-mannopyranose + D-mannopyranose | - |
? | |
alpha-(1-3)-mannobiose + H2O | highest activity and affinity | Aspergillus sp. | alpha-D-mannopyranose + D-mannopyranose | - |
? | |
alpha-(1-6)-mannobiose | low activity | Aspergillus sp. | alpha-D-mannopyranose + D-mannopyranose | - |
? | |
alpha-(1-6)-mannobiose + H2O | low activity | Aspergillus sp. | alpha-D-mannopyranose | - |
? | |
Man6-glycopeptide + H2O | - |
Aspergillus sp. | alpha-D-mannopyranose + ? | - |
? | |
Man9-glycopeptide + H2O | - |
Aspergillus sp. | alpha-D-mannopyranose + ? | - |
? | |
p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Aspergillus sp. | p-nitrophenol + alpha-D-mannopyranose | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 69200, SDS-PAGE | Aspergillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
stable for 16 h | Aspergillus sp. |
50 | - |
stable for 30 min at pH 6.0 | Aspergillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 6.5 | - |
Aspergillus sp. |