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Literature summary for 3.2.1.24 extracted from

  • Gaikwald, S.M.; Keskar, S.S.; Khan, M.I.
    Purification and characterization of alpha-D-mannosidase from Aspergillus sp. (1995), Biochim. Biophys. Acta, 1250, 144-148.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-mannopyranose 55% inhibition at 30 mM Aspergillus sp.
Co2+ 46% inhibition at 1 mM Aspergillus sp.
Cu2+ complete inhibition at 1 mM Aspergillus sp.
D-glucose 67% inhibition at 30 mM Aspergillus sp.
Fe2+ 54% inhibition at 1 mM Aspergillus sp.
Hg2+ complete inhibition at 1 mM Aspergillus sp.
Mn2+ slight inhibition at 1 mM Aspergillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.083
-
p-nitrophenyl-alpha-D-mannopyranoside
-
Aspergillus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
69200
-
6 * 69200, SDS-PAGE Aspergillus sp.
412000
-
gel filtration Aspergillus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alpha-(1-2)-mannobiose + H2O Aspergillus sp.
-
alpha-D-mannopyranose + D-mannopyranose
-
?
alpha-(1-3)-mannobiose + H2O Aspergillus sp. highest activity and affinity alpha-D-mannopyranose + D-mannopyranose
-
?
alpha-(1-6)-mannobiose Aspergillus sp. low activity alpha-D-mannopyranose + D-mannopyranose
-
?
Man6-glycopeptide + H2O Aspergillus sp.
-
alpha-D-mannopyranose + ?
-
?
Man9-glycopeptide + H2O Aspergillus sp.
-
alpha-D-mannopyranose + ?
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 3.8% neutral sugar content Aspergillus sp.

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, preparative PAGE Aspergillus sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Aspergillus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.36
-
-
Aspergillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-(1-2)-mannobiose + H2O
-
Aspergillus sp. alpha-D-mannopyranose + D-mannopyranose
-
?
alpha-(1-3)-mannobiose + H2O highest activity and affinity Aspergillus sp. alpha-D-mannopyranose + D-mannopyranose
-
?
alpha-(1-6)-mannobiose low activity Aspergillus sp. alpha-D-mannopyranose + D-mannopyranose
-
?
alpha-(1-6)-mannobiose + H2O low activity Aspergillus sp. alpha-D-mannopyranose
-
?
Man6-glycopeptide + H2O
-
Aspergillus sp. alpha-D-mannopyranose + ?
-
?
Man9-glycopeptide + H2O
-
Aspergillus sp. alpha-D-mannopyranose + ?
-
?
p-nitrophenyl-alpha-D-mannopyranoside + H2O
-
Aspergillus sp. p-nitrophenol + alpha-D-mannopyranose
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 69200, SDS-PAGE Aspergillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
stable for 16 h Aspergillus sp.
50
-
stable for 30 min at pH 6.0 Aspergillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
Aspergillus sp.