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Literature summary for 3.2.1.24 extracted from
- Purification and properties of alpha-D-mannosidase from germinated seeds of Medicago sativa (alfalfa) (1980), Biochem. J., 185, 455-462.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Medicago sativa |  |
| Cd2+ | - |
Medicago sativa | |
| Cu2+ | - |
Medicago sativa | |
| EDTA | addition of Zn2+ restores inhibitory effect | Medicago sativa | |
| Fe2+ | - |
Medicago sativa | |
| Fe3+ | - |
Medicago sativa | |
| Hg2+ | - |
Medicago sativa | |
| Zn2+ | - |
Medicago sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
p-nitrophenyl-alpha-D-mannopyranoside | - |
Medicago sativa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE | Medicago sativa |
| 50000 | - |
1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE | Medicago sativa |
| 60000 | - |
1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE | Medicago sativa |
| 75000 | - |
1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE | Medicago sativa |
| 230000 | - |
gel filtration, SDS-PAGE | Medicago sativa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Medicago sativa | - |
alfalfa | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps, preparative electrophoresis | Medicago sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Medicago sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 215 | - |
- |
Medicago sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl-alpha-D-mannopyranoside + H2O | - |
Medicago sativa | p-nitrophenol + alpha-D-mannopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 1 * 75000 + 1 * 60000 + 1 * 50000 + 1 * 45000, SDS-PAGE | Medicago sativa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
thermal activation up to | Medicago sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | - |
- |
Medicago sativa |
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