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Literature summary for 3.2.1.25 extracted from
- Biochemical characterization of a recombinant thermostable beta-mannosidase from Thermotoga maritima with transglycosidase activity (2009), J. Mol. Catal. B, 60, 119-124 .
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, activation to 112% of control | Thermotoga maritima |  |
| Ca2+ | 1 mM, activation to 108% of control | Thermotoga maritima | |
| dithiothreitol | 1 mM, activation to 154% of control | Thermotoga maritima | |
| EDTA | 1 mM, activation to 102% of control | Thermotoga maritima | |
| Fe2+ | 1 mM, activation to 105% of control | Thermotoga maritima | |
| K+ | 1 mM, activation to 193 % of control | Thermotoga maritima | |
| Li+ | 1 mM, activation to 187% of control | Thermotoga maritima | |
| N-bromosuccinimide | 1 mM, activation to 177% of control | Thermotoga maritima | |
| Na+ | 1 mM, activation to 180% of control | Thermotoga maritima | |
| Ni2+ | 1 mM, activation to 144% of control | Thermotoga maritima | |
| SDS | 1 mM, activation to 148% of control | Thermotoga maritima | |
| Sr2+ | 1 mM, activation to 203% of control | Thermotoga maritima | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as a soluble C-terminal His-tagged protein in Escherichia coli | Thermotoga maritima |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 98% loss of activity | Thermotoga maritima | |
| Co2+ | 1 mM, 12% loss of activity | Thermotoga maritima | |
| Cu2+ | 1 mM, 72% loss of activity | Thermotoga maritima | |
| Hg2+ | 1 mM, 98% loss of activity | Thermotoga maritima | |
| Mg2+ | 1 mM, 4% loss of activity | Thermotoga maritima | |
| Mn2+ | 1 mM, 4% loss of activity | Thermotoga maritima | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.49 | - |
4-nitrophenyl beta-D-mannopyranoside | pH 7.0, 70°C | Thermotoga maritima | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 93200 | - |
gel filtration | Thermotoga maritima |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermotoga maritima | Q9X1V9 | - |
- |
| Thermotoga maritima DSM 3109 | Q9X1V9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermotoga maritima |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 37.2 | - |
substrate: 4-nitrophenyl beta-D-mannopyranoside, pH 7.0, 70°C | Thermotoga maritima |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Thermotoga maritima | 4-nitrophenol + beta-D-mannose | - |
? | |
| 4-nitrophenyl beta-D-mannopyranoside + H2O | - |
Thermotoga maritima DSM 3109 | 4-nitrophenol + beta-D-mannose | - |
? | |
| mannobiose + H2O | activity is 15% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside | Thermotoga maritima | 2 D-mannopyranose | - |
? | |
| mannobiose + H2O | activity is 15% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside | Thermotoga maritima DSM 3109 | 2 D-mannopyranose | - |
? | |
| mannotetraose + H2O | activity is 32% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside | Thermotoga maritima | 4 D-mannopyranose | - |
? | |
| mannotetraose + H2O | activity is 32% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside | Thermotoga maritima DSM 3109 | 4 D-mannopyranose | - |
? | |
| mannotriose + H2O | activity is 47% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside | Thermotoga maritima | 3 D-mannopyranose | - |
? | |
| mannotriose + H2O | activity is 47% compared to the activity with 4-nitrophenyl beta-D-mannopyranoside | Thermotoga maritima DSM 3109 | 3 D-mannopyranose | - |
? | |
| additional information | the enzyme also exhibited transglycosidase activity when the reactionwas carried out with 10% (w/v) of mannobiose in the presence of alcohols or galactose | Thermotoga maritima | ? | - |
? | |
| additional information | the enzyme also exhibited transglycosidase activity when the reactionwas carried out with 10% (w/v) of mannobiose in the presence of alcohols or galactose | Thermotoga maritima DSM 3109 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 96800, SDS-PAGE | Thermotoga maritima |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TM1624 | - |
Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 95 | - |
- |
Thermotoga maritima |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | 100 | 80°C: about 65% of maximal activity, 100°C: 68% of maximal activity | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life: 828 min | Thermotoga maritima |
| 85 | - |
half-life: 213 min | Thermotoga maritima |
| 90 | - |
half-life: 189 min | Thermotoga maritima |
| 95 | - |
half-life: 28 min | Thermotoga maritima |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermotoga maritima |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | pH 4.0: about 50% of maximal activity, pH 10.0: about 40% of maximal activity | Thermotoga maritima |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 70°C, 30 minm, retention of 80% of maximal activity between the range of pH 5.0 and 8.0 | Thermotoga maritima |
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