Any feedback?
Literature summary for 3.2.1.26 extracted from
- Purification and characterization of invertase from Lactobacillus reuteri CRL 1100 (2000), Curr. Microbiol., 40, 181-184.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 10 mM, 47% increase in activity | Limosilactobacillus reuteri |  |
| iodoacetamide | enhances activity | Limosilactobacillus reuteri | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Limosilactobacillus reuteri | |
| Ca2+ | - |
Limosilactobacillus reuteri | |
| Cd2+ | 1 mM, 66% inhibition | Limosilactobacillus reuteri | |
| Cu2+ | 1 mM, 90% inhibition | Limosilactobacillus reuteri | |
| dithiothreitol | - |
Limosilactobacillus reuteri | |
| Hg2+ | 1 mM, 67% inhibition | Limosilactobacillus reuteri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.66 | - |
sucrose | - |
Limosilactobacillus reuteri | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Limosilactobacillus reuteri | - |
CRL 1100 | - |
| Limosilactobacillus reuteri CRL 1100 | - |
CRL 1100 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 48% carbohydrate | Limosilactobacillus reuteri |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Limosilactobacillus reuteri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Limosilactobacillus reuteri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| raffinose + H2O | - |
Limosilactobacillus reuteri | fructose + melibiose | - |
? | |
| raffinose + H2O | - |
Limosilactobacillus reuteri CRL 1100 | fructose + melibiose | - |
? | |
| stachyose + H2O | - |
Limosilactobacillus reuteri | ? | - |
? | |
| stachyose + H2O | - |
Limosilactobacillus reuteri CRL 1100 | ? | - |
? | |
| sucrose + H2O | - |
Limosilactobacillus reuteri | D-fructose + D-glucose | - |
? | |
| sucrose + H2O | - |
Limosilactobacillus reuteri CRL 1100 | D-fructose + D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Limosilactobacillus reuteri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 45 | 20°C: about 80% of maximal activity, 45°C: about 70% of maximal activity | Limosilactobacillus reuteri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 4.5-7.0, stable below | Limosilactobacillus reuteri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Limosilactobacillus reuteri |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | pH 4.5: about 35% of maximal activity, pH 7.0: about 45% of maximal activity | Limosilactobacillus reuteri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | 45°C, stable | Limosilactobacillus reuteri |
| 5 | 6 | stable | Limosilactobacillus reuteri |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
