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Literature summary for 3.2.1.26 extracted from
- Purification and characterization of an invertase produced by Aspergillus ochraceus TS (2001), Indian J. Biochem. Biophys., 38, 180-185.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd-acetate | 10 mM, 50% inhibition | Aspergillus ochraceus |  |
| Hg2+ | 1 mM, complete inhibition | Aspergillus ochraceus | |
| NEM | 10 mM, complete inhibition | Aspergillus ochraceus | |
| PCMB | 10 mM, 90% inhibition | Aspergillus ochraceus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
sucrose | pH 5.0, 40°C | Aspergillus ochraceus | |
| 4.5 | - |
raffinose | pH 5.0, 40°C | Aspergillus ochraceus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus ochraceus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | activates | Aspergillus ochraceus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 66000 | - |
2 * 66000, SDS-PAGE | Aspergillus ochraceus |
| 130000 | - |
gel filtration | Aspergillus ochraceus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus ochraceus | - |
- |
- |
| Aspergillus ochraceus TS | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | 30% of the total mass of the enzyme comprises of neutral sugar, contains mannose | Aspergillus ochraceus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus ochraceus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture filtrate | - |
Aspergillus ochraceus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 160.4 | - |
- |
Aspergillus ochraceus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no hydrolysis of maltose, cellobiose and lactose | Aspergillus ochraceus | ? | - |
? | |
| additional information | no hydrolysis of maltose, cellobiose and lactose | Aspergillus ochraceus TS | ? | - |
? | |
| raffinose | 96% of the activity with sucrose | Aspergillus ochraceus | beta-D-fructose + melibiose | - |
? | |
| raffinose | 96% of the activity with sucrose | Aspergillus ochraceus TS | beta-D-fructose + melibiose | - |
? | |
| sucrose + H2O | - |
Aspergillus ochraceus | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Aspergillus ochraceus TS | beta-D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 66000, SDS-PAGE | Aspergillus ochraceus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Aspergillus ochraceus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 55 | - |
Aspergillus ochraceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Aspergillus ochraceus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | - |
Aspergillus ochraceus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus ochraceus | - |
- |
4.2 |
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