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Literature summary for 3.2.1.26 extracted from
- Characterization of a purified beta-fructofuranosidase from Bifidobacterium infantis ATCC 15697 (2002), Lett. Appl. Microbiol., 35, 462-467.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 1 mM, 26% inhibition of beta-fructofuranosidase activity, no effect on invertase activity | Bifidobacterium longum subsp. infantis |  |
| Cu2+ | 1 mM, 75% inhibition of beta-fructofuranosidase activity, no effect on invertase activity | Bifidobacterium longum subsp. infantis | |
| Fe2+ | 1 mM, 9% inhibition of beta-fructofuranosidase activity, 7% inhibition of invertase activity | Bifidobacterium longum subsp. infantis | |
| Hg2+ | 1 mM, complete inhibition of beta-fructofuranosidase activity, 65% inhibition of invertase activity | Bifidobacterium longum subsp. infantis | |
| Mn2+ | 1 mM, 1% inhibition of fructofuranosidase activity | Bifidobacterium longum subsp. infantis | |
| PCMB | 1 mM, 80% inhibition of beta-fructofuranosidase activity, 65% inhibition of invertase activity | Bifidobacterium longum subsp. infantis | |
| Zn2+ | 1 mM, 26% inhibition of beta-fructofuranosidase activity, 13% inhibition of invertase activity | Bifidobacterium longum subsp. infantis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Bifidobacterium longum subsp. infantis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| intracellular | - |
Bifidobacterium longum subsp. infantis | 5622 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | 1 mM, activation of invertase activity to 115% of control | Bifidobacterium longum subsp. infantis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
1 * 70000, SDS-PAGE | Bifidobacterium longum subsp. infantis |
| 72000 | - |
gel filtration | Bifidobacterium longum subsp. infantis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum subsp. infantis | - |
ATCC 15697 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bifidobacterium longum subsp. infantis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 580 | - |
- |
Bifidobacterium longum subsp. infantis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| fructooligosaccharide + H2O | - |
Bifidobacterium longum subsp. infantis | D-fructose + ? | - |
? | |
| inulin | 6% of the activity with fructooligosaccharides | Bifidobacterium longum subsp. infantis | D-fructose | - |
? | |
| sucrose + H2O | 10% of the activity with fructooligosaccharides | Bifidobacterium longum subsp. infantis | beta-D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 70000, SDS-PAGE | Bifidobacterium longum subsp. infantis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Bifidobacterium longum subsp. infantis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 45 | 25°C: about 45% of maximal activity, 45°C: about 45% of maximal activity, fructofuranosidase activity | Bifidobacterium longum subsp. infantis |
| 25 | 55 | 25°C: about 45% of maximal activity, 50°C: about 90% of maximal activity, 55°C: about 25% of maximal activity, invertase activity | Bifidobacterium longum subsp. infantis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
50% loss of beta-fructofuranosidase activity after 30 min, 50% loss of invertase activity after 75 min | Bifidobacterium longum subsp. infantis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
fructofuranosidase and invertase activity | Bifidobacterium longum subsp. infantis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.5 | pH 4.5: about 50% of maximal activity of fructofuranosidase and invertase activity, pH 7.5: about 50% of maximal activity of invertase activity, about 40% of maximal activity of fructofuranosidase activity | Bifidobacterium longum subsp. infantis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bifidobacterium longum subsp. infantis | chromatofocusing | - |
4.2 |
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