General Stability | Organism |
---|---|
polyol activated enzyme is stable up to 72 hours | Cyberlindnera jadinii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sorbitol | inhibitory action at 30 and 40% solution | Cyberlindnera jadinii | |
xylitol | inhibitory action at 35 and 40% solution | Cyberlindnera jadinii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 3.5 | sucrose | native invertase, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 | Cyberlindnera jadinii | |
10.05 | - |
sucrose | 25% xylitol, 55°C, pH 4.5 | Cyberlindnera jadinii | |
10.75 | - |
sucrose | 30% glycerol, 55°C, pH 4.5 | Cyberlindnera jadinii | |
11.5 | - |
sucrose | 15% sorbitol, 55°C, pH 4.5 | Cyberlindnera jadinii | |
12.5 | - |
sucrose | native invertase, 55°C, pH 4.5 | Cyberlindnera jadinii | |
16.66 | - |
sucrose | 40% xylitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 | Cyberlindnera jadinii | |
17.85 | - |
sucrose | 40% glycerol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 | Cyberlindnera jadinii | |
19.23 | - |
sucrose | 40% glycerol, 55°C, pH 9 | Cyberlindnera jadinii | |
21.27 | - |
sucrose | 40% sorbitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 | Cyberlindnera jadinii | |
21.73 | - |
sucrose | 40% xylitol, 55°C, pH 9 | Cyberlindnera jadinii | |
29.41 | - |
sucrose | native invertase, 55°C, pH 9 | Cyberlindnera jadinii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | Cyberlindnera jadinii | - |
D-fructose + D-glucose | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Glycerol | no significant increase in alpha-helical content of invertase with 30% glycerol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-6% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 79, 82, 85, and 84 degrees Celsius with 10, 20, 30, and 40% glycerol, stability increase due to hydration | Cyberlindnera jadinii |
additional information | with sorbitol no significant increase in alpha-helical content of invertase with 20% sorbitol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-5%, at pH 9 increases of alpha-helical content from 3-6% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 77, 79, 81, and 82 degrees Celsius with 10, 20, 30, 40% sorbitol, respectively, and from 70 to 72 and 73 degrees Celsius with 30 and 40% sorbitol, respectively, at pH 9, stability increase due to hydration | Cyberlindnera jadinii |
additional information | xylitol with no significant increase in alpha-helical content of invertase with 25% xylitol at optimal conditions, under pre-incubation at 80 degrees Celsius for 20 min 30% concentration increases alpha-helical content from 2-6%, at pH 9 increases of alpha-helical content from 3-7% and tertiary structure is conserved under both conditions, increase of thermal denaturation temperature from 75 to 79, 82, and 84 degrees Celsius with 10, 20, and 30-40% xylitol and from 70 to 73, 75, 77 degrees Celsius with 20, 30, 40% xylitol at pH 9, stability increase due to hydration | Cyberlindnera jadinii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
maximum activity at 15% sorbitol (1.23-fold increase), increased activity from 5-20% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5 | Cyberlindnera jadinii |
additional information | - |
maximum activity at 25% xylitol (1.57-fold increase), increased activity from 10-30% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5 | Cyberlindnera jadinii |
additional information | - |
maximum activity at 30% glycerol (1.39-fold increase), increased activity from 10-40% solutions, 100 mM sucrose, 10 min, 55°C, 50 mM sodium acetate buffer, pH 4.5 | Cyberlindnera jadinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + H2O | - |
Cyberlindnera jadinii | D-fructose + D-glucose | - |
? | |
sucrose + H2O | 100 mM sucrose, 10 min, 55 degrees Celsius, 50 mM sodium acetate buffer, pH 4.5 | Cyberlindnera jadinii | D-fructose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
invertase | - |
Cyberlindnera jadinii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, pH 9.0, thermal denaturation starts without solvent or with 20% sorbitol | Cyberlindnera jadinii |
72 | - |
20 min, pH 9.0, thermal denaturation starts with 30% sorbitol | Cyberlindnera jadinii |
73 | - |
20 min, pH 9.0, thermal denaturation starts with 20% xylitol or 40% sorbitol | Cyberlindnera jadinii |
75 | - |
20 min, pH 4.5, thermal denaturation starts without solvent | Cyberlindnera jadinii |
75 | - |
20 min, pH 9.0, thermal denaturation starts with 30% xylitol | Cyberlindnera jadinii |
77 | - |
20 min, pH 4.5, thermal denaturation starts with 10% sorbitol | Cyberlindnera jadinii |
77 | - |
20 min, pH 9.0, thermal denaturation starts with 40% xylitol | Cyberlindnera jadinii |
79 | - |
20 min, pH 4.5, thermal denaturation starts with 10% glycerol or xylitol, or 20% sorbitol | Cyberlindnera jadinii |
80 | - |
20 min, pH 4.5, 70% residual activity retained with 40% glycerol | Cyberlindnera jadinii |
80 | - |
20 min, pH 4.5, 80% residual activity with 40% xylitol | Cyberlindnera jadinii |
80 | - |
20 min, pH 4.5, maximum of 45% residual activity above 20% to 40% sorbitol | Cyberlindnera jadinii |
81 | - |
20 min, pH 4.5, thermal denaturation starts with 30% sorbitol | Cyberlindnera jadinii |
82 | - |
20 min, pH 4.5, thermal denaturation starts with 20% glycerol or xylitol, or 40% sorbitol | Cyberlindnera jadinii |
84 | - |
20 min, pH 4.5, thermal denaturation starts with 30% and 40% xylitol or 40% glycerol | Cyberlindnera jadinii |
85 | - |
20 min, pH 4.5, thermal denaturation starts with 30% glycerol | Cyberlindnera jadinii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
93.3 | - |
sucrose | native invertase, 55°C, pH 9 | Cyberlindnera jadinii | |
154 | - |
sucrose | native invertase, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 | Cyberlindnera jadinii | |
690.8 | - |
sucrose | 40% sorbitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 | Cyberlindnera jadinii | |
737.5 | - |
sucrose | 40% xylitol, 55°C, pH 9 | Cyberlindnera jadinii | |
1055 | - |
sucrose | 40% glycerol, 55°C, pH 9 | Cyberlindnera jadinii | |
1078 | - |
sucrose | 40% glycerol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 | Cyberlindnera jadinii | |
1232 | - |
sucrose | 40% xylitol, pre-incubation at 80°C for 20 min, 55°C, pH 4.5 | Cyberlindnera jadinii | |
1541 | - |
sucrose | native invertase, 55°C, pH 4.5 | Cyberlindnera jadinii | |
1891 | - |
sucrose | 15% sorbitol, 55°C, pH 4.5 | Cyberlindnera jadinii | |
2148 | - |
sucrose | 30% glycerol, 55°C, pH 4.5 | Cyberlindnera jadinii | |
2428 | - |
sucrose | 25% xylitol, 55°C, pH 4.5 | Cyberlindnera jadinii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
9 | - |
94% activity loss of native enzyme | Cyberlindnera jadinii |
9 | - |
no protecting effect of glycerol | Cyberlindnera jadinii |
9 | - |
sorbitol retains 48% activity at 40% concentration | Cyberlindnera jadinii |
9 | - |
xylitol retains 68% activity at 40% concentration | Cyberlindnera jadinii |