Any feedback?
Literature summary for 3.2.1.26 extracted from
- Characterization of recombinant beta-fructofuranosidase from Bifidobacterium adolescentis G1 (2010), Chem. Cent. J., 4, 09.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| deoxycholic acid | 10 mM, 137% of initial activity activity | Bifidobacterium adolescentis |  |
| Triton X-100 | 10 mM, 138% of initial activity activity | Bifidobacterium adolescentis | |
| Tween-80 | 10 mM, 138% of initial activity activity | Bifidobacterium adolescentis | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bifidobacterium adolescentis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 10 mM, no residual activity | Bifidobacterium adolescentis | |
| Cu2+ | 10 mM, 52% residual activity | Bifidobacterium adolescentis | |
| Hg2+ | 10 mM, no residual activity | Bifidobacterium adolescentis | |
| Sodium dodecyl sulfate | 10 mM, no residual activity | Bifidobacterium adolescentis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.7 | - |
1-kestose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 1.7 | - |
neokestose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 2.2 | - |
fructosylnystose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 3.9 | - |
nystose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 25.9 | - |
inulin | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 38 | - |
sucrose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60300 | - |
x * 66000, SDS-PAGE, x * 60300, MALDI TOF-MS | Bifidobacterium adolescentis |
| 66000 | - |
x * 66000, SDS-PAGE, x * 60300, MALDI TOF-MS | Bifidobacterium adolescentis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium adolescentis | - |
- |
- |
| Bifidobacterium adolescentis G1 | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 86 | - |
pH 5.7, 37°C | Bifidobacterium adolescentis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-kestose + H2O | - |
Bifidobacterium adolescentis | D-fructose + ? | - |
? | |
| 1-kestose + H2O | - |
Bifidobacterium adolescentis G1 | D-fructose + ? | - |
? | |
| fructosylnystose + H2O | - |
Bifidobacterium adolescentis | D-fructose + ? | - |
? | |
| inulin + H2O | - |
Bifidobacterium adolescentis | D-fructose + ? | - |
? | |
| inulin + H2O | - |
Bifidobacterium adolescentis G1 | D-fructose + ? | - |
? | |
| neokestose + H2O | - |
Bifidobacterium adolescentis | ? | - |
? | |
| neokestose + H2O | - |
Bifidobacterium adolescentis G1 | ? | - |
? | |
| nystose + H2O | - |
Bifidobacterium adolescentis | D-fructose + ? | - |
? | |
| nystose + H2O | - |
Bifidobacterium adolescentis G1 | D-fructose + ? | - |
? | |
| sucrose + H2O | - |
Bifidobacterium adolescentis | beta-D-fructose + alpha-D-glucose | - |
? | |
| sucrose + H2O | - |
Bifidobacterium adolescentis G1 | beta-D-fructose + alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 66000, SDS-PAGE, x * 60300, MALDI TOF-MS | Bifidobacterium adolescentis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
stable up to | Bifidobacterium adolescentis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 75.4 | - |
fructosylnystose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 77.4 | - |
inulin | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 79.4 | - |
sucrose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 107 | - |
1-kestose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 142.7 | - |
neokestose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 152.8 | - |
nystose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | - |
- |
Bifidobacterium adolescentis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.6 | - |
Bifidobacterium adolescentis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.1 | - |
sucrose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 3 | - |
inulin | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 34.2 | - |
fructosylnystose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 39.2 | - |
nystose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 63.2 | - |
1-kestose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
| 83.9 | - |
neokestose | pH 5.7, 37°C | Bifidobacterium adolescentis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
