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Literature summary for 3.2.1.28 extracted from
- Purification and properties of alpha,alpha-trehalase from the mucosa of rat small intestine (1975), Biochim. Biophys. Acta, 403, 139-146.
General Stability
| General Stability | Organism |
|---|---|
| rapid inactivation during butanol treatment at temperatures above 4°C for periods of more than 3 min at 4°C | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cellobiose | 10 mM, 60% inhibition | Rattus norvegicus |  |
| Hg2+ | 0.1 mM, complete inhibition | Rattus norvegicus | |
| Mg2+ | 1 mM MgCl2, 30% inhibition | Rattus norvegicus | |
| PCMB | 1 mM, 30% inhibition | Rattus norvegicus | |
| sucrose | 10 mM, 60% inhibition | Rattus norvegicus | |
| Tris | 10 mM, complete inhibition | Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.4 | - |
trehalose | - |
Rattus norvegicus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
1 * 90000, SDS-PAGE | Rattus norvegicus |
| 96000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| intestine | mucosa of small intestine | Rattus norvegicus | - |
| intestine | small intestine | Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 127 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| trehalose + H2O | absolute specificity | Rattus norvegicus | D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 90000, SDS-PAGE | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 5.7 | - |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | 7 | pH 3.2: about 50% of maximal activity, pH 7.0: about 60% of maximal activity | Rattus norvegicus |
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Release 2023.1 (January 2023)
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