Literature summary for 3.2.1.28 extracted from

Kadowaki, M.K.; de Lourdes Polizeli, M.; Terenzi, H.F.; Jorge, J.A.
Characterization of trehalase activities from the thermophilic fungus Scytalidium thermophilum (1996), Biochim. Biophys. Acta, 1291, 199-205.

Search for more literature for 3.2.1.28

General Stability

General Stability	Organism
Ca2+ and Mn2+ protect from thermoinactivation	Mycothermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
ADP	-	Mycothermus thermophilus
Al3+	1 mM AlCl3, complete inhibition	Mycothermus thermophilus
ATP	-	Mycothermus thermophilus
Cu2+	1 mM CuSO4, complete inhibition of extracellular enzyme, 47% inhibition of intracellular enzyme	Mycothermus thermophilus
EDTA	1 mM, 30% inhibition of extracellular enzyme, 54% inhibition of intracellular enzyme	Mycothermus thermophilus
Hg2+	1 mM HgCl2, complete inhibition	Mycothermus thermophilus
NH4Cl	1 mM NH4Cl, 22% inhibition of extracellular enzyme, 27% inhibition of intracellular enzyme	Mycothermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.24	-	trehalose	intracellular enzyme	Mycothermus thermophilus
3.58	-	trehalose	extracellular enzyme	Mycothermus thermophilus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	intracellular and extracellular enzyme	Mycothermus thermophilus	-	-
intracellular	intracellular and extracellular enzyme	Mycothermus thermophilus	5622	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	activates	Mycothermus thermophilus
Co2+	slight stimulation	Mycothermus thermophilus
Mn2+	activates	Mycothermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
82000	-	5 * 82000, extracellular enzyme, SDS-PAGE	Mycothermus thermophilus
85000	-	5 * 85000, intracellular enzyme, SDS-PAGE	Mycothermus thermophilus
370000	-	extracellular enzyme, gel filtration	Mycothermus thermophilus
398000	-	intracellular enzyme, gel filtration	Mycothermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Mycothermus thermophilus	-	intracellular and extracellular threhalase	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	extracellular enzyme contains 81% carbohydrate, intracellular enzyme contains 51% carbohydrate	Mycothermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1775	-	extracellular enzyme	Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
trehalose + H2O	absolute specificity	Mycothermus thermophilus	D-glucose	-	?

Subunits

Subunits	Comment	Organism
pentamer	5 * 82000, extracellular enzyme, SDS-PAGE	Mycothermus thermophilus
pentamer	5 * 85000, intracellular enzyme, SDS-PAGE	Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	extracellular enzyme	Mycothermus thermophilus
65	-	intracellular enzyme	Mycothermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	extracellular enzyme has a half-life of 3 min. Intracellular enzyme loses 20% of activity after 40 min when incubated without Ca2+, and is fully stable in presence of Ca2+	Mycothermus thermophilus
65	-	intracellular enzyme has a half-life of 4.5 min when incubated without Ca2+	Mycothermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	extracellular and intracellular enzyme	Mycothermus thermophilus

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Release 2023.1 (January 2023)