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Literature summary for 3.2.1.28 extracted from

  • De Almeida, E.M.; de Lourdes Polizeli, M.; Terenzi, H.F.; Jorge, J.A.
    Biochemical characterization of a Ca2+-dependent acid trehalase activity from the thermophilic fungus Chaetomium thermophilum var. coprophilum (1999), FEMS Microbiol. Lett., 171, 11-15.
No PubMed abstract available
Search for more literature for 3.2.1.28

Inhibitors

Inhibitors Comment Organism Structure
ADP 1 mM, 53% inhibition Thermochaetoides thermophila
Al3+ 1 mM, complete inhibition Thermochaetoides thermophila
ATP 1 mM, 80% inhibition Thermochaetoides thermophila
barium ion 1 mM, complete inhibition Thermochaetoides thermophila
Cu2+ 1 mM, complete inhibition Thermochaetoides thermophila
EDTA 1 mM, 55% inhibition Thermochaetoides thermophila
Hg2+ 1 mM, complete inhibition Thermochaetoides thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
 trehalose presence of Ca2+ Thermochaetoides thermophila

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Thermochaetoides thermophila
Co2+ stimulates Thermochaetoides thermophila
Mn2+ stimulates Thermochaetoides thermophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
98000
-
 2 * 98000, SDS-PAGE Thermochaetoides thermophila
200000
-
 gel filtration Thermochaetoides thermophila

Organism

Organism UniProt Comment Textmining
Thermochaetoides thermophila
-
 var. coprophilum
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein no carbohydrate detected Thermochaetoides thermophila

Purification (Commentary)

Purification (Comment) Organism
-
 Thermochaetoides thermophila

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
120
-
-
 Thermochaetoides thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
trehalose + H2O
-
 Thermochaetoides thermophila D-glucose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 98000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermochaetoides thermophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60 50°C: 39% of maximal activity, 60°C: 44% of maximal activity Thermochaetoides thermophila

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 addition of Ca2+ decreases stability, half-life at 65°C is diminished about 2fold Thermochaetoides thermophila
50
-
 30 min, stable up to Thermochaetoides thermophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Thermochaetoides thermophila