Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | 10 mM, in presence of CaCl2, 18% residual activity for extracellular isoform, 12% residual activity, intracellular isoform. No activation in absence of Ca2+ | Mycothermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 10 mM, 16% residual activity, extracellular isoform, 12% residual activity, intracellular isoform. In presence of CaCl2, 18% residual activity for extracellular isoform, 12% residual activity, intracellular isoform, respectively | Mycothermus thermophilus | |
Al3+ | 1 mM, no residual activity, both isoforms | Mycothermus thermophilus | |
ATP | 10 mM, 12% residual activity, extracellular isoform, 6% residual activity, intracellular isoform. In presence of CaCl2, 2% residual activity for extracellular isoform, 10% residual activity, intracellular isoform | Mycothermus thermophilus | |
EDTA | 1 mM, 70% residual activity for extracellular isoform, 46% residual activity, intracellular isoform | Mycothermus thermophilus | |
Hg2+ | 1 mM, no residual activity, both isoforms | Mycothermus thermophilus | |
Zn2+ | 1 mM, 50% residual activity for extracellular isoform, not inhibitory for intracellular isoform | Mycothermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.24 | - |
alpha,alpha-trehalose | intracellular isoform, pH 6.0, 65°C | Mycothermus thermophilus | |
3.09 | - |
alpha,alpha-trehalose | presence of Mn2+, intracellular isoform, pH 6.0, 65°C | Mycothermus thermophilus | |
3.58 | - |
alpha,alpha-trehalose | extracellular isoform, pH 6.0, 60°C | Mycothermus thermophilus | |
6.43 | - |
alpha,alpha-trehalose | presence of Ca2+, intracellular isoform, pH 6.0, 65°C | Mycothermus thermophilus | |
7.63 | - |
alpha,alpha-trehalose | presence of Mn2+, extracellular isoform, pH 6.0, 60°C | Mycothermus thermophilus | |
9.51 | - |
alpha,alpha-trehalose | presence of Ca2+, extracellular isoform, pH 6.0, 60°C | Mycothermus thermophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Mycothermus thermophilus | - |
- |
intracellular | - |
Mycothermus thermophilus | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM, 2.5fold stimulation, both isoforms | Mycothermus thermophilus | |
Mn2+ | 5 mM, 3fold stimulation, both isoforms | Mycothermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
370000 | - |
gel filtration, extracellular isoform | Mycothermus thermophilus |
392000 | - |
gel filtration, intracellular isoform | Mycothermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content 81%, extracellular isoform, and 51%, intracellular isoform, respectively | Mycothermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1700 | - |
extracellular isoform, pH 6.0, 60°C | Mycothermus thermophilus |
3700 | - |
intracellular isoform, pH 6.0, 65°C | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha,alpha-trehalose + H2O | both isoforms are highly specific for trehalose | Mycothermus thermophilus | 2 D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | 5 * 82000, extracellular isoform, 5 * 85000, intracellular isoform, SDS-PAGE | Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
extracellular isoform | Mycothermus thermophilus |
65 | - |
intracellular isoform | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
both isoforms | Mycothermus thermophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycothermus thermophilus | isoelectric focusing, intracellular isoform | - |
3.4 |
Mycothermus thermophilus | isoelectric focusing, extracellular isoform | - |
3.7 |