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Literature summary for 3.2.1.28 extracted from

  • Kadowaki, M.K.; Polizeli, M.L.; Terenzi, H.F.; Jorge, J.A.
    Characterization of trehalase activities from the thermophilic fungus Scytalidium thermophilum (1996), Biochim. Biophys. Acta, 1291, 199-205 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
AMP 10 mM, in presence of CaCl2, 18% residual activity for extracellular isoform, 12% residual activity, intracellular isoform. No activation in absence of Ca2+ Mycothermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
ADP 10 mM, 16% residual activity, extracellular isoform, 12% residual activity, intracellular isoform. In presence of CaCl2, 18% residual activity for extracellular isoform, 12% residual activity, intracellular isoform, respectively Mycothermus thermophilus
Al3+ 1 mM, no residual activity, both isoforms Mycothermus thermophilus
ATP 10 mM, 12% residual activity, extracellular isoform, 6% residual activity, intracellular isoform. In presence of CaCl2, 2% residual activity for extracellular isoform, 10% residual activity, intracellular isoform Mycothermus thermophilus
EDTA 1 mM, 70% residual activity for extracellular isoform, 46% residual activity, intracellular isoform Mycothermus thermophilus
Hg2+ 1 mM, no residual activity, both isoforms Mycothermus thermophilus
Zn2+ 1 mM, 50% residual activity for extracellular isoform, not inhibitory for intracellular isoform Mycothermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.24
-
alpha,alpha-trehalose intracellular isoform, pH 6.0, 65°C Mycothermus thermophilus
3.09
-
alpha,alpha-trehalose presence of Mn2+, intracellular isoform, pH 6.0, 65°C Mycothermus thermophilus
3.58
-
alpha,alpha-trehalose extracellular isoform, pH 6.0, 60°C Mycothermus thermophilus
6.43
-
alpha,alpha-trehalose presence of Ca2+, intracellular isoform, pH 6.0, 65°C Mycothermus thermophilus
7.63
-
alpha,alpha-trehalose presence of Mn2+, extracellular isoform, pH 6.0, 60°C Mycothermus thermophilus
9.51
-
alpha,alpha-trehalose presence of Ca2+, extracellular isoform, pH 6.0, 60°C Mycothermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Mycothermus thermophilus
-
-
intracellular
-
Mycothermus thermophilus 5622
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 5 mM, 2.5fold stimulation, both isoforms Mycothermus thermophilus
Mn2+ 5 mM, 3fold stimulation, both isoforms Mycothermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
370000
-
gel filtration, extracellular isoform Mycothermus thermophilus
392000
-
gel filtration, intracellular isoform Mycothermus thermophilus

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein carbohydrate content 81%, extracellular isoform, and 51%, intracellular isoform, respectively Mycothermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1700
-
extracellular isoform, pH 6.0, 60°C Mycothermus thermophilus
3700
-
intracellular isoform, pH 6.0, 65°C Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha,alpha-trehalose + H2O both isoforms are highly specific for trehalose Mycothermus thermophilus 2 D-glucose
-
?

Subunits

Subunits Comment Organism
pentamer 5 * 82000, extracellular isoform, 5 * 85000, intracellular isoform, SDS-PAGE Mycothermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
extracellular isoform Mycothermus thermophilus
65
-
intracellular isoform Mycothermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
both isoforms Mycothermus thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Mycothermus thermophilus isoelectric focusing, intracellular isoform
-
3.4
Mycothermus thermophilus isoelectric focusing, extracellular isoform
-
3.7