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Literature summary for 3.2.1.3 extracted from

  • Basaveswara Rao, V.; Sastri, N.V.S.; Subba Rao, P.V.
    Purification and characterization of a thermostable glucoamylase from the thermophilic fungus Thermomyces lanuginosus (1981), Biochem. J., 193, 379-387.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.3

General Stability

General Stability Organism
after treatment with 4 M guanidinium chloride, gel filtration on Sephadex G-25 column results in 50% loss of activity Thermomyces lanuginosus
after treatment with 8 M urea, gel filtration on Sephadex G-25 column results in complete recovery of activity Thermomyces lanuginosus
stable to treatment with 1.5% SDS Thermomyces lanuginosus

Inhibitors

Inhibitors Comment Organism Structure
D-glucono-1,5-lactone non-competitive Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Thermomyces lanuginosus
1.1
-
 maltotriose
-
 Thermomyces lanuginosus
2.75
-
 p-nitrophenyl alpha-D-glucoside
-
 Thermomyces lanuginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 1 * 55000, SDS-PAGE Thermomyces lanuginosus
57000
-
 gel filtration Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
 ML-M
-
Thermomyces lanuginosus ML-M
-
 ML-M
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 10-12% carbohydrate Thermomyces lanuginosus

Purification (Commentary)

Purification (Comment) Organism
-
 Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80
-
-
 Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucoside + H2O
-
 Thermomyces lanuginosus 4-nitrophenol + D-glucose
-
 ?
amylopectin + H2O
-
 Thermomyces lanuginosus D-glucose + ?
-
 ?
amylopectin + H2O
-
 Thermomyces lanuginosus ML-M D-glucose + ?
-
 ?
dextrin + H2O
-
 Thermomyces lanuginosus ?
-
 ?
dextrin + H2O
-
 Thermomyces lanuginosus ML-M ?
-
 ?
glycogen + H2O
-
 Thermomyces lanuginosus glucose + ?
-
 ?
glycogen + H2O
-
 Thermomyces lanuginosus ML-M glucose + ?
-
 ?
maltose + H2O
-
 Thermomyces lanuginosus 2 glucose
-
 ?
maltotriose + H2O hydrolysis by a multi-chain mechanism Thermomyces lanuginosus maltose + glucose
-
 ?
additional information no hydrolysis of alpha-1,6 linkages Thermomyces lanuginosus ?
-
 ?
additional information no hydrolysis of alpha-1,6 linkages Thermomyces lanuginosus ML-M ?
-
 ?
starch + H2O soluble Thermomyces lanuginosus glucose + ?
-
 ?
starch + H2O soluble Thermomyces lanuginosus ML-M glucose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 55000, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 6 h, completly stable Thermomyces lanuginosus
60
-
 pH 6.0, 6 h, about 40% loss of activity, half-life: 7.3 h Thermomyces lanuginosus
70
-
 pH 6.0, half-life: 30 min Thermomyces lanuginosus
80
-
 pH 6.0, half-life: 10 min Thermomyces lanuginosus