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Literature summary for 3.2.1.3 extracted from
- Purification and characterization of a thermostable glucoamylase from Aspergillus fumigatus (1998), Can. J. Microbiol., 44, 493-497.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 10 mM, strong inhibition | Aspergillus fumigatus |  |
| additional information | remarkable insensitivity of the enzyme to end product inhibition | Aspergillus fumigatus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Aspergillus fumigatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 23% carbohydrate | Aspergillus fumigatus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus fumigatus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 150 | - |
- |
Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | no activity | Aspergillus fumigatus | glucose + ? | - |
? | |
| amylopectin + H2O | 85% of activity with starch | Aspergillus fumigatus | D-glucose + ? | - |
? | |
| amylose + H2O | 93% of the activity with starch | Aspergillus fumigatus | glucose + ? | - |
? | |
| glycogen + H2O | 82% of the activity with starch | Aspergillus fumigatus | glucose + ? | - |
? | |
| isomaltose + H2O | 10% of the activity with starch | Aspergillus fumigatus | glucose | - |
? | |
| maltose + H2O | 40% of the activity with starch | Aspergillus fumigatus | 2 glucose | - |
? | |
| maltotriose + H2O | 46% of the activity with starch | Aspergillus fumigatus | maltose + glucose | - |
? | |
| pullulan + H2O | 52% of the activity with starch | Aspergillus fumigatus | ? | - |
? | |
| starch + H2O | soluble | Aspergillus fumigatus | glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Aspergillus fumigatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
half-life in absence of starch: 15 min. Half-life in presene of 3% w/v starch or sorbitol: 50 min. Half-life in presence of 10% sorbitol: 40 min. Half-life in presence of 30% sorbitol: 65 min | Aspergillus fumigatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 4.5 | - |
Aspergillus fumigatus |
| 4.5 | 5.5 | - |
Aspergillus fumigatus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 7 | 40°C, 2 days, stable | Aspergillus fumigatus |
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