Protein Variants | Comment | Organism |
---|---|---|
A246C | the T50-value is enhanced by 4°C to 73°C. Compared to wild-type enzyme, the mutant is twice as active at 66°C but half as active at 45°C | Aspergillus awamori |
C320A | barely improved thermostability or altered activity | Aspergillus awamori |
P307A/T310V/Y312M/N313G | up to 15fold decreased turnover-number for alpha-1,4-linked substrates. Up to 9fold increase in Km-value for alpha-1,6-linked substrates | Aspergillus awamori |
V181T/N182Y/G183A | 2fold increased Km-value for alpha-1,4-linked substrates: For alpha-1,6-linked substrates a 2fold increase in Km and a 3fold decrease in turnover-number | Aspergillus awamori |
V181T/N182Y/G183A/P307A/T310V/Y312M/A313G | remarkably low Km-value for isomaltotriose through isomaltoheptaose and elevated turnover-number on isomaltose, resulting in an approximately 2fold improved catalytic effeciency | Aspergillus awamori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values for the wild type enzyme and the mutant enzymes V181T/N182Y/G183A, P307A/T310V/Y312M/N313G and V181T/N182Y/G183A/P307A/T310V/Y312M/N313G | Aspergillus awamori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus awamori | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isomaltoheptaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
isomaltohexaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
isomaltopentaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
isomaltose + H2O | - |
Aspergillus awamori | glucose | - |
? | |
isomaltotetraose + H2O | - |
Aspergillus awamori | ? | - |
? | |
isomaltotriose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltoheptaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltohexaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltopentaose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltose + H2O | - |
Aspergillus awamori | 2 glucose | - |
? | |
maltotetraose + H2O | - |
Aspergillus awamori | ? | - |
? | |
maltotriose + H2O | - |
Aspergillus awamori | maltose + glucose | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers for the wild-type enzyme and the mutant enzymes V181T/N182Y/G183A, P307A/T310V/Y312M/N313G and V181T/N182Y/G183A/P307A/T310V/Y312M/N313G | Aspergillus awamori |