KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
starch | 1 mg/ml, 50°C, pH 4.0 | Sclerotinia sclerotiorum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 50% increase in activity | Sclerotinia sclerotiorum | |
Mn2+ | 1 mM, 20% increase in activity | Sclerotinia sclerotiorum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72000 | - |
x * 72000, SDS-PAGE | Sclerotinia sclerotiorum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylose + H2O | Sclerotinia sclerotiorum | 13% of activity with starch | beta-D-glucose + ? | - |
? | |
glycogen + 3 H2O | Sclerotinia sclerotiorum | 150% of activity with starch | 4 beta-D-glucose | - |
? | |
starch + H2O | Sclerotinia sclerotiorum | - |
beta-D-glucose + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sclerotinia sclerotiorum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Sclerotinia sclerotiorum |
Purification (Comment) | Organism |
---|---|
ammonium sulfate, G-25 Sephadex, Mono Q, Econo-Pac S, TSK 2000 Gel | Sclerotinia sclerotiorum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl alpha-D-glucopyranoside + H2O | 12% of activity with starch | Sclerotinia sclerotiorum | 4-nitrophenol + D-glucose | - |
? | |
amylose + H2O | 13% of activity with starch | Sclerotinia sclerotiorum | beta-D-glucose + ? | - |
? | |
glycogen + 3 H2O | 150% of activity with starch | Sclerotinia sclerotiorum | 4 beta-D-glucose | - |
? | |
starch + H2O | - |
Sclerotinia sclerotiorum | beta-D-glucose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 72000, SDS-PAGE | Sclerotinia sclerotiorum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sclerotinia sclerotiorum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | 4 | - |
Sclerotinia sclerotiorum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sclerotinia sclerotiorum | isoelectric focusing, probably 4 to 5 isoforms with pIs from 4.8 to 5.4 | - |
6 |