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Literature summary for 3.2.1.3 extracted from
- Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain (2006), FEBS J., 273, 2161-2171.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain AH22, secretion of recombinant enzymes | Saccharomycopsis fibuligera |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant nonglycosylated enzyme, 10 mg/ml protein in 50 mM acetate, pH 5.4, with 15% PEG 8000, X-ray diffraction structure determination and analysis at 1.1-1.6 A resolution, modelling | Saccharomycopsis fibuligera |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H447A | site-directed mutagenesis, structure analysis compared to the wild-type, crystal structure | Saccharomycopsis fibuligera |
| H447A/D450A | site-directed mutagenesis, structure analysis compared to the wild-type, crystal structure | Saccharomycopsis fibuligera |
| R15A | site-directed mutagenesis, structure analysis compared to the wild-type, crystal structure | Saccharomycopsis fibuligera |
| T462A | site-directed mutagenesis, structure analysis compared to the wild-type, crystal structure | Saccharomycopsis fibuligera |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | enzyme secretion | Saccharomycopsis fibuligera | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Saccharomycopsis fibuligera | - |
starch + beta-D-glucose | - |
? |  |
| starch + H2O | Saccharomycopsis fibuligera HUT 7212 | - |
starch + beta-D-glucose | - |
? | |
| starch + H2O | Saccharomycopsis fibuligera IFO 0111 | - |
starch + beta-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomycopsis fibuligera | P08017 | Glu1 precursor | - |
| Saccharomycopsis fibuligera | Q8TFE5 | Glu-1.1 precursor | - |
| Saccharomycopsis fibuligera HUT 7212 | P08017 | Glu1 precursor | - |
| Saccharomycopsis fibuligera IFO 0111 | Q8TFE5 | Glu-1.1 precursor | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Saccharomycopsis fibuligera |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant enzymes from Saccharomyces cerevisiae strain AH22 medium by gel filtration and ion exchange chromatography to homogeneity | Saccharomycopsis fibuligera |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose | active site structure, catalytic residue is Tyr464, catalytic domain and raw starch binding site | Saccharomycopsis fibuligera |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Saccharomycopsis fibuligera | starch + beta-D-glucose | - |
? | |
| starch + H2O | - |
Saccharomycopsis fibuligera HUT 7212 | starch + beta-D-glucose | - |
? | |
| starch + H2O | - |
Saccharomycopsis fibuligera IFO 0111 | starch + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | the enzyme consists of a catalytic domain and a starch binding domain connected by an O-glycosylated peptide linker located at the N-terminus, the enzyme contains 7 subsites for substrate binding | Saccharomycopsis fibuligera |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-1,4-D-glucan glucohydrolase | - |
Saccharomycopsis fibuligera |
| Glu-1.1 | - |
Saccharomycopsis fibuligera |
| Glu-A | - |
Saccharomycopsis fibuligera |
| Glu1 | - |
Saccharomycopsis fibuligera |
| Glucan 1,4-alpha-glucosidase | - |
Saccharomycopsis fibuligera |
| glucoamylase | - |
Saccharomycopsis fibuligera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
assay at | Saccharomycopsis fibuligera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.6 | - |
assay at | Saccharomycopsis fibuligera |
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Release 2023.1 (January 2023)
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