Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain MV1184 | Thermoactinomyces vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
W622C | site-directed mutagenesis, the mutant enzyme shows slightly altered pH optimum and 87% reduced activity compared to the wild-type enzyme | Thermoactinomyces vulgaris |
W622D | site-directed mutagenesis, the mutant enzyme shows slightly altered pH optimum and 95% reduced activity compared to the wild-type enzyme | Thermoactinomyces vulgaris |
W622G | site-directed mutagenesis, the mutant enzyme shows slightly altered pH optimum and 95.7% reduced activity compared to the wild-type enzyme | Thermoactinomyces vulgaris |
W622H | site-directed mutagenesis, the mutant enzyme shows slightly altered pH optimum and 48% reduced activity compared to the wild-type enzyme | Thermoactinomyces vulgaris |
W622S | site-directed mutagenesis, the mutant enzyme shows slightly altered pH optimum and 83% reduced activity compared to the wild-type enzyme | Thermoactinomyces vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces vulgaris | - |
- |
- |
Thermoactinomyces vulgaris R-47 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-1-alpha-D-glucopyranose + beta-D-glucopyranose | residue Trp622 is important in substrate binding rather than in determination of pH optimum, and probably does not interact with the catalytic base | Thermoactinomyces vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltotetraose + H2O | - |
Thermoactinomyces vulgaris | maltotriose + beta-D-glucose | - |
? | |
maltotetraose + H2O | - |
Thermoactinomyces vulgaris R-47 | maltotriose + beta-D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Thermoactinomyces vulgaris |
tGA | - |
Thermoactinomyces vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Thermoactinomyces vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
30 min, mutants W622C and W622S, stable | Thermoactinomyces vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
optimum of mutant W622D | Thermoactinomyces vulgaris |
6.4 | - |
optimum of mutant W622G | Thermoactinomyces vulgaris |
6.5 | - |
optimum of wild-type enzyme | Thermoactinomyces vulgaris |
6.6 | - |
optimum of mutants W622C and W622S | Thermoactinomyces vulgaris |
6.8 | - |
optimum of mutant W622H | Thermoactinomyces vulgaris |