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Literature summary for 3.2.1.3 extracted from
- Kinetics of enhanced ethanol productivity using raw starch hydrolyzing glucoamylase from Aspergillus niger mutant produced in solid state fermentation (2004), Lett. Appl. Microbiol., 39, 13-18.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | the enzyme of the M115 mutant strain is useful for enhanced ethanol production by Saccharomyces cerevisiae, strain ATTC26602, using raw starch as substrate in solid state fermentation | Aspergillus niger |
| synthesis | the enzyme of the M115 mutant strain is useful for enhanced ethanol production by Saccharomyces cerevisiae, strain ATTC26602, using raw starch as substrate in solid state fermentation | Aspergillus niger |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics and thermodynamics of wild-type and mutant enzymes | Aspergillus niger |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
x * 90000, wild-type and mutant enzymes | Aspergillus niger |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Aspergillus niger | hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the starch chain, substrates corn cobs, maize starch, soluble starch, and wheat bran | starch + beta-D-glucose | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
wild-type strain NIAB 280 and M115 mutant | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | hydrolysis of terminal 1,4-linked alpha-D-glucose residues successively from non-reducing ends of the starch chain, substrates corn cobs, maize starch, soluble starch, and wheat bran | Aspergillus niger | starch + beta-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, wild-type and mutant enzymes | Aspergillus niger |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GA | - |
Aspergillus niger |
| glucoamylase | - |
Aspergillus niger |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
wild-type enzyme | Aspergillus niger |
| 60 | - |
mutant enzyme | Aspergillus niger |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life of wild-type enzyme is 3.6 h, of mutant enzyme 7.3 h | Aspergillus niger |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | mutant enzyme | Aspergillus niger |
| 5.5 | - |
wild-type enzyme | Aspergillus niger |
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Release 2023.1 (January 2023)
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