KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.74 | - |
maltodextrin | pH 4.5, 60°C, native enzyme | Aspergillus niger | |
3.64 | - |
maltodextrin | pH 4.5, 60°C, glycated enzyme | Aspergillus niger | |
9.19 | - |
maltose | pH 4.5, 60°C, glycated enzyme | Aspergillus niger | |
12.49 | - |
maltose | pH 4.5, 60°C, native enzyme | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77600 | - |
x * 77600, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by ion exchange and hydrophobic interaction chromatography | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
61.34 | - |
glycated purified enzyme | Aspergillus niger |
65.64 | - |
native purified enzyme | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltodextrin + H2O | during incubation 5-(hydroxymethyl)-2-furfuraldehyde is released, resulting in a glycated enzyme | Aspergillus niger | maltodextrin + beta-D-glucose | - |
? | |
maltose + H2O | - |
Aspergillus niger | beta-D-glucose + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 77600, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
rapid loss of activity, purified enzyme | Aspergillus niger |
6.5 | - |
inactivation, purified enzyme | Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | native and glycated enzyme | - |
3.5 |