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Literature summary for 3.2.1.3 extracted from
- Improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain (2008), Appl. Microbiol. Biotechnol., 77, 1225-1232.
Application
| Application | Comment | Organism |
|---|---|---|
| industry | improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain, overview | Rhizopus arrhizus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae strain MT8-1 on the cell surface, subcloning in Escherichia coli | Rhizopus arrhizus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | use of cell surface engineering to display Rhizopus oryzae glucoamylase on the cell surface of yeast Saccharomyces cerevisiae, improvement in enzymatic desizing of starched cotton cloth using yeast codisplaying glucoamylase and cellulose-binding domain, overview | Rhizopus arrhizus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Rhizopus arrhizus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Rhizopus arrhizus | glucoamylase is an exo-amylolytic enzyme that cleaves alpha-1,4-linked and alpha-1,6-linked glucose from starch | beta-D-glucose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus arrhizus | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme activity of different recombinant constructs in Saccharomyces cerevisiae cultures, overview | Rhizopus arrhizus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Rhizopus arrhizus | beta-D-glucose + ? | - |
? | |
| starch + H2O | glucoamylase is an exo-amylolytic enzyme that cleaves alpha-1,4-linked and alpha-1,6-linked glucose from starch | Rhizopus arrhizus | beta-D-glucose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Rhizopus arrhizus |
| More | the enzyme belongs to the family 15 glycoside hydrolase | Rhizopus arrhizus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rhizopus arrhizus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Rhizopus arrhizus |
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Release 2023.1 (January 2023)
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