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Literature summary for 3.2.1.3 extracted from
- Characterization of a novel glucoamylase from the thermoacidophilic archaeon Picrophilus torridus heterologously expressed in E. coli (2006), Eng. Life Sci., 6, 311-317.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| phylogenetic tree, expression of the intracellular enzyme in Escherichia coli | Picrophilus torridus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.9 | - |
maltotriose | pH 5.0, 50°C, recombinant enzyme | Picrophilus torridus |  |
| 4.9 | - |
maltose | pH 5.0, 50°C, recombinant enzyme | Picrophilus torridus | |
| 9.9 | - |
maltotetraose | pH 5.0, 50°C, recombinant enzyme | Picrophilus torridus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Picrophilus torridus | - |
- |
| intracellular | - |
Picrophilus torridus | 5622 | - |
| additional information | the extracellular enzyme contains a signal sequence | Picrophilus torridus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73000 | - |
4 * 73000, intracellular enzyme, SDS-PAGE | Picrophilus torridus |
| 92000 | - |
x * 92000, extracellular enzyme | Picrophilus torridus |
| 292000 | - |
recombinant intracellular enzyme, native PAGE | Picrophilus torridus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Picrophilus torridus | Q6KYX5 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | the extracellular enzyme contains a signal sequence | Picrophilus torridus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant intracellular enzyme 37fold from Escherichia coli by heat treatment at 70°C for 30 min, anion exchange chromatography, and gel filtration to homogeneity | Picrophilus torridus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | optimal growth at pH 0.7 and 60 °C | Picrophilus torridus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | potato amylopectin | Picrophilus torridus | D-glucose + ? | - |
? | |
| amylose + H2O | potato amylose type III | Picrophilus torridus | D-glucose + ? | - |
? | |
| dextrin + 6 H2O | - |
Picrophilus torridus | 7 D-glucose | - |
? | |
| maltose + H2O | - |
Picrophilus torridus | 2 D-glucose | - |
? | |
| maltotetraose + H2O | - |
Picrophilus torridus | maltotriose + D-glucose | - |
? | |
| maltotriose + H2O | - |
Picrophilus torridus | maltose + D-glucose | - |
? | |
| additional information | the intracellular enzyme shows a unique substrate specificity compared to already known glucoamylases, in addition to the hydrolysis of branched and linear alpha-glucans, the purified enzyme preferentially attacks maltotriose, overview, oligossaccharides Dp2, Dp4, Dp5, Dp6, and Dp7 as well as isomaltose, panose, and isopanose are poor substrates | Picrophilus torridus | ? | - |
? | |
| starch + H2O | - |
Picrophilus torridus | D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 92000, extracellular enzyme | Picrophilus torridus |
| tetramer | 4 * 73000, intracellular enzyme, SDS-PAGE | Picrophilus torridus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucoamylase | - |
Picrophilus torridus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
intracellular enzyme | Picrophilus torridus |
| 90 | - |
extracellular enzyme | Picrophilus torridus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | intracellular enzyme | Picrophilus torridus |
| 50 | 100 | extracellular enzyme | Picrophilus torridus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
purified recombinant intracellular enzyme, 48 h, stable | Picrophilus torridus |
| 55 | - |
purified recombinant intracellular enzyme, half-life is 8 h | Picrophilus torridus |
| 60 | - |
purified recombinant intracellular enzyme, half-life is 1 h | Picrophilus torridus |
| 65 | - |
purified recombinant intracellular enzyme, inctivation within 5 min | Picrophilus torridus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2 | - |
extracellular enzyme | Picrophilus torridus |
| 5 | - |
intracellular enzyme | Picrophilus torridus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
- |
4 | extracellular enzyme | Picrophilus torridus |
| 4.5 | 6.5 | intracellular enzyme | Picrophilus torridus |
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