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Literature summary for 3.2.1.3 extracted from

  • Bhatti, H.N.; Rashid, M.H.; Nawaz, R.; Asgher, M.; Perveen, R.; Jabbar, A.
    Purification and characterization of a novel glucoamylase from Fusarium solani (2007), Food Chem., 103, 338-343.
    View publication on PubMed
Search for more literature for 3.2.1.3

Inhibitors

Inhibitors Comment Organism Structure
Fe3+
-
 Fusarium solani
Hg2+ strong inhibition Fusarium solani
Ni2+
-
 Fusarium solani
Pb2+ strong inhibition Fusarium solani
Zn2+ strong inhibition Fusarium solani

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Fusarium solani
1.9
-
 soluble starch pH 4.5, 40°C, purified native enzyme Fusarium solani

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Fusarium solani
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ slight activation Fusarium solani
Mg2+ slight activation Fusarium solani

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 1 * 40000, SDS-PAGE Fusarium solani
41000
-
 gel filtration Fusarium solani

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Fusarium solani important industrial enzyme that removes the glucose units from the non-reducing chain-ends of starch and glycogen by hydrolyzing alpha-1,4 linkages consecutively D-glucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Fusarium solani
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 26.2fold to homogeneity by ammonium sulfate precipitation, anion exchange chromatography, and hydrophobic interaction chromatography, followed by gel filtration Fusarium solani

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Fusarium solani

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
soluble starch + H2O
-
 Fusarium solani beta-D-glucose + ?
-
 ?
soluble starch + H2O
-
 Fusarium solani beta-D-glucose + soluble starch
-
 ?
starch + H2O soluble starch Fusarium solani D-glucose + ?
-
 ?
starch + H2O important industrial enzyme that removes the glucose units from the non-reducing chain-ends of starch and glycogen by hydrolyzing alpha-1,4 linkages consecutively Fusarium solani D-glucose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 40000, SDS-PAGE Fusarium solani

Synonyms

Synonyms Comment Organism
glucoamylase
-
 Fusarium solani

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 assay at Fusarium solani

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 60
-
 Fusarium solani

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified native enzyme, 79% remaining activity after 60 min Fusarium solani
60
-
 purified native enzyme, half-life is 26 min Fusarium solani

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
7.35
-
 soluble starch pH 4.5, 40°C, purified native enzyme Fusarium solani

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Fusarium solani

pH Range

pH Minimum pH Maximum Comment Organism
2 9
-
 Fusarium solani