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Literature summary for 3.2.1.3 extracted from

  • Perveen, R.; Rashid, M.H.; Saleem, M.; Khalid, A.M.; Rajoka, M.I.
    Kinetic and thermodynamic properties of an immobilized glucoamylase from a mesophilic fungus, Arachniotus citrinus (2006), Protein Pept. Lett., 13, 665-671.
    View publication on PubMed
Search for more literature for 3.2.1.3

Application

Application Comment Organism
biotechnology enzyme immobilization on polyacrylamide gel results in an enzyme with increases thermostability for use in biocatalysis Gymnascella citrina
synthesis enzyme immobilization on polyacrylamide gel results in an enzyme with increases thermostability for use in biocatalysis Gymnascella citrina

Protein Variants

Protein Variants Comment Organism
additional information enzyme immobilization on polyacrylamide gel highly decreases the entropy and enthalpy of thermal enzyme deactivation Gymnascella citrina

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics and thermodynamics of polyacrylamide gel-immobilized purified enzyme Gymnascella citrina
0.031
-
 starch immobilized enzyme, pH 5.5, 90°C Gymnascella citrina
0.1
-
 starch free enzyme, pH 5.5, 90°C Gymnascella citrina
0.12
-
 starch immobilized enzyme, pH 5.5, 70°C Gymnascella citrina
0.18
-
 starch free enzyme, pH 5.5, 70°C Gymnascella citrina
0.238
-
 starch immobilized enzyme, pH 5.5, 50°C Gymnascella citrina
0.333
-
 starch free enzyme, pH 5.5, 50°C Gymnascella citrina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Gymnascella citrina
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gymnascella citrina
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 63.3fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography, followed by another step of anion exchange chromatography to homogeneity Gymnascella citrina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Gymnascella citrina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
 Gymnascella citrina ?
-
 ?

Synonyms

Synonyms Comment Organism
glucoamylase
-
 Gymnascella citrina

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 free enzyme Gymnascella citrina
60
-
 immobilized enzyme Gymnascella citrina

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 90 temperature profile of free and immobilized enzyme, overview Gymnascella citrina

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 immobilized enzyme, stable up to, melting temperature of the free enzyme Gymnascella citrina
70
-
 melting temperature of the immobilized enzyme Gymnascella citrina

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
 starch immobilized enzyme, pH 5.5, 50°C Gymnascella citrina
4.12
-
 starch free enzyme, pH 5.5, 50°C Gymnascella citrina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 free enzyme Gymnascella citrina
5.5
-
 immobilized enzyme Gymnascella citrina

pH Range

pH Minimum pH Maximum Comment Organism
3 11 pH profile of free and immobilized enzyme, overview Gymnascella citrina