Application | Comment | Organism |
---|---|---|
industry | glucoamylase from Aspergillus niger is an industrially important biocatalyst that is utilized in the mass production of glucose from raw starch or soluble oligosaccharides | Aspergillus niger |
Crystallization (Comment) | Organism |
---|---|
purified isolated N-terminal catalytic domain of the G1 isoform, generated by subtilisin cleavage, sitting-drop vapour-diffusion method, 20 mg/ml protein at pH 8.5, 22°C room temperatur, the reservoir solution contains 50 mM Tris acetate, pH 8.5, 22.5% PEG 6000, 0.4 M sodium acetate and 10% glycerol, mixing of 0.001 ml protein and reservoir solution and equilibration against 1 ml reservoir solution, 2 weeks, X-ray diffraction structure determination and analysis at 1.9 A resolution, the active site of the enzyme is in complex with both Tris and glycerol molecules, structure modelling | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | P69328 | G1 isoform | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the linker region between starch-binding and catalytic domains is heavily glycosylated. The O-glycosylated residues are Ser467(443), Ser468(444), Thr476(452), Ser477(453), Ser483(459), Ser484(460) and Thr486(462) | Aspergillus niger |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Aspergillus niger |
More | the G1 isoform consists of a catalytic domain and a starch-binding domain connected by a heavily glycosylated linker region | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
glucoamylase | - |
Aspergillus niger |
General Information | Comment | Organism |
---|---|---|
physiological function | glucoamylase is an exoglucohydrolase that primarily catalyzes the hydrolysis of alpha-1,4 glycosidic linkages in raw starch and soluble oligosaccharides to generate beta-D-glucose | Aspergillus niger |